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However, the effect of endogenous H<jats:sub>2<\/jats:sub>S on the development of nasopharyngeal carcinoma (NPC) is still unknown. In this study, aminooxyacetic acid (AOAA, an inhibitor of cystathionine\u2010\u03b2\u2010synthase), <jats:sc>dl<\/jats:sc>\u2010propargylglycine (PAG, an inhibitor of cystathionine\u2010\u03b3\u2010lyase), and <jats:sc>l<\/jats:sc>\u2010aspartic acid (<jats:sc>l<\/jats:sc>\u2010Asp, an inhibitor of 3\u2010mercaptopyruvate sulfurtransferase) were adopted to detect the role of endogenous H<jats:sub>2<\/jats:sub>S in NPC growth. The results indicated that the combine (PAG\u2009+\u2009AOAA\u2009+\u2009<jats:sc>l<\/jats:sc>\u2010Asp) group had higher inhibitory effect on the growth of NPC cells than the PAG, AOAA, and <jats:sc>l<\/jats:sc>\u2010Asp groups. There were similar trends in the levels of apoptosis and reactive oxygen species (ROS). In addition, the combine group exhibited lower levels of phospho (p)\u2010extracellular signal\u2010regulated protein kinase but higher expressions of p\u2010p38 and p\u2010c\u2010Jun N\u2010terminal kinase than those in the AOAA, PAG, and <jats:sc>l<\/jats:sc>\u2010Asp groups. Furthermore, the combine group exerted more potent inhibitory effect on NPC xenograft tumor growth without obvious toxicity. In summary, suppression of endogenous H<jats:sub>2<\/jats:sub>S generation could dramatically inhibit NPC growth via the ROS\/mitogen\u2010activated protein kinase pathway. Endogenous H<jats:sub>2<\/jats:sub>S may be a novel therapeutic target in human NPC cells. Effective inhibitors for H<jats:sub>2<\/jats:sub>S\u2010producing enzymes could be designed and developed for NPC treatment.<\/jats:p>","DOI":"10.1002\/mc.23513","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T12:10:04Z","timestamp":1675858204000},"page":"652-664","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of endogenous hydrogen sulfide production suppresses the growth of nasopharyngeal carcinoma cells"],"prefix":"10.1002","volume":"62","author":[{"given":"Da\u2010Yong","family":"Wang","sequence":"first","affiliation":[{"name":"Henan International Joint Laboratory for Nuclear Protein Regulation, School of Basic Medical Sciences Henan University Kaifeng Henan China"},{"name":"The First Affiliated Hospital of Henan University Kaifeng Henan 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