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The goal of the study here was to examine if C\u2010X\u2010C motif chemokine 12 (CXCL12) in BC cells could manipulate protumorigenic properties of tumor\u2010associated macrophages (TAMs) which affects anticancer immunity supporting tumor development in the tumor microenvironment. CXCL12 was found to be overexpressed in BC and predicted poor survival. CXCL12 in BC was associated with multiple immune cell infiltrations, with TAM infiltration playing a key role. CXCL12 elevated chemotaxis of TAMs. CXCL12 downregulation inhibited cellular activity and TAM and suppressed the ability of TAMs to secrete inflammatory factors and MMP9. Furthermore, chromatin immunoprecipitation analysis revealed that SPI1 was localized to the CXCL12 promoter in BC cells, suggesting that CXCL12 serves a direct target of SPI1, which was consistent with the fact that SPI1 reversed the repressive effects of si\u2010CXCL12 on BC cell activity and TAM recruitment in vitro and in vivo. Collectively, these findings suggest that SPI1 is involved in modulating TAM recruitment, representing a new mechanism through which it may influence tumor growth. This may be partly mediated by regulating CXCL12 expression.<\/jats:p>","DOI":"10.1002\/mc.23663","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T15:31:56Z","timestamp":1701444716000},"page":"448-460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["SPI1\u2010mediated CXCL12 expression in bladder cancer affects the recruitment of tumor\u2010associated macrophages"],"prefix":"10.1002","volume":"63","author":[{"given":"Guimei","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Laboratory Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute Shenyang Liaoning People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Qiu","sequence":"additional","affiliation":[{"name":"Medical Oncology Department of Gastrointestinal Cancer Cancer Hospital of China Medical University, Liaoning Cancer 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