{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T01:01:25Z","timestamp":1790557285201,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Golgi phosphoprotein 3 (GOLPH3) has been reported as an oncogene in various tumors; however, the role and function of GOLPH3 and its relevant molecular mechanism in cholangiocarcinoma (CCA) are unclear. Herein, GOLPH3 expression in CCA tissues was observed to be significantly higher than that in paired adjacent noncancerous tissues. Clinicopathological analysis showed that GOLPH3 expression correlated positively with the tumor\u2010node\u2010metastasis stage. In addition, GOLPH3 expression correlated inversely with the overall survival of patients with CCA. Multivariate analysis showed that GOLPH3 was an independent prognostic factor for patients with CCA. Transcriptome analysis (RNA sequencing) of <jats:italic>GOLPH3<\/jats:italic> knockdown cells showed that the expression levels of nine ferroptosis\u2010related genes were significantly changed, indicating the important biological function of GOLPH3 in ferroptosis in CCA cells. Furthermore, <jats:italic>GOLPH3<\/jats:italic> knockdown could significantly promote Erastin\u2010induced ferroptosis in vitro and suppress tumor growth <jats:italic>in viv<\/jats:italic>o. Overexpression of <jats:italic>GOLPH3<\/jats:italic> had the opposite effect on this phenotype. Further studies revealed that <jats:italic>GOLPH3<\/jats:italic> knockdown was significantly associated with a decrease in cysteine content, an accumulation of the lipid peroxidation product malondialdehyde, an increase in reactive oxygen species, and sensitized CCA cells to Erastin\u2010induced ferroptosis. Moreover, changes in GOLPH3 expression were found to be consistent with the expression of light chain subunit solute carrier family 7 member 11 (SLC7A11). Thus, our study suggested that GOLPH3 functions as an oncoprotein in CCA and may suppress ferroptosis by facilitating SLC7A11 expression, suggesting that GOLPH3 could serve as a therapeutic target for CCA treatment.<\/jats:p>","DOI":"10.1002\/mc.23697","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T10:26:41Z","timestamp":1708684001000},"page":"912-925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["GOLPH3 promotes tumor malignancy via inhibition of ferroptosis by upregulating SLC7A11 in cholangiocarcinoma"],"prefix":"10.1002","volume":"63","author":[{"given":"Zheng","family":"Yin","sequence":"first","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pancreatic<sup>\u2212<\/sup>Biliary Surgery The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunling","family":"Qi","sequence":"additional","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianzhou","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Pancreatic<sup>\u2212<\/sup>Biliary Surgery The First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meifang","family":"He","sequence":"additional","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5262-1317","authenticated-orcid":false,"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of General Surgery Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61903-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.131"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.01.035"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21759"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08109"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-3081"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2010.10.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21030933"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2019.2804"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-3156"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00324-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.021"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.10.014"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.04.002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/3877617"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.158"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2022\/4250531","article-title":"Eukaryotic translation initiation factor 5A independently predicts poor prognosis of cholangiocarcinoma patients and regulates the ferroptosis and mitochondrial apoptosis","volume":"2022","author":"Wan P","year":"2022","journal-title":"J Oncol"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.15462"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15531"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-0310-z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0363-5"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v13.i9.1003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/14656566.2021.2010707"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-022-02462-9"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.575358"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.808152"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045622"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.124"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i48.13473"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14344"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8120637"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-00462-0"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201847563"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2021.8064"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.03.079"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.23697","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T12:32:50Z","timestamp":1713357170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.23697"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,23]]},"references-count":36,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1002\/mc.23697"],"URL":"https:\/\/doi.org\/10.1002\/mc.23697","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"value":"0899-1987","type":"print"},{"value":"1098-2744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,23]]},"assertion":[{"value":"2023-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}