{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:30:58Z","timestamp":1790667058463,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\u03b2\u2010elemene has a variety of anti\u2010inflammatory, antioxidant, and antitumor effects. Currently, the influence of \u03b2\u2010elemene on adrenocortical carcinoma (ACC) malignant progression and action mechanism remains unclear. This research aims to explore the influence and action mechanism of \u03b2\u2010elemene on ACC progression. The impacts of \u03b2\u2010elemene on ACC cell viability, proliferation, migration, and apoptosis were investigated through CCK\u20108 assay, clone formation assay, Transwell experiment, Wound healing assay, and flow cytometry. The miR\u2010486\u20103p expression was analyzed utilizing RT\u2010qPCR. According to different databases, neuronal pentraxin 1 (NPTX1) is the predicted downstream target gene of miR\u2010486\u20103p. Western blot and RT\u2010qPCR were utilized to examine NPTX1 expression. Silencing miR\u2010486\u20103p or Overexpression NPTX1 in ACC cells further explored whether \u03b2\u2010elemene affects ACC cells by regulating miR\u2010486\u20103p\/NPTX1. Finally, a subcutaneous graft tumor model was constructed to investigate how \u03b2\u2010elemene may impact tumor growth in vivo. \u03b2\u2010elemene decreased the cell viability, hindered cell proliferation and migration capacity, and induced apoptosis of ACC cells. miR\u2010486\u20103p level in ACC cells was notably reduced in comparison to normal cells, but treatment with \u03b2\u2010elemene markedly increased miR\u2010486\u20103p expression. Additionally, ACC cells showed high level of NPTX1, while miR\u2010486\u20103p targeted negative regulation of NPTX1. Overexpression miR\u2010486\u20103p hindered the malignant progression of ACC cells, whereas overexpression NPTX1 reversed the impact of overexpression miR\u2010486\u20103p. Silencing miR\u2010486\u20103p or overexpression NPTX1 both attenuated the suppressive influence of \u03b2\u2010elemene on the malignant behavior of ACC cells. Additionally, tumor growth was suppressed and apoptosis was induced in tumor cells in vivo by \u03b2\u2010elemene. In conclusion, \u03b2\u2010elemene reduces ACC cell viability, hinders proliferation and migration, and induces apoptosis through the miR\u2010486\u20103p\/NPTX1 axis.<\/jats:p>","DOI":"10.1002\/mc.23879","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:59:27Z","timestamp":1736765967000},"page":"691-702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["\u03b2\u2010Elemene Inhibits Adrenocortical Carcinoma Cell Proliferation and Migration, and Induces Apoptosis by Up\u2010Regulating miR\u2010486\u20103p\/Targeting NPTX1 Axis"],"prefix":"10.1002","volume":"64","author":[{"given":"Yan","family":"Lin","sequence":"first","affiliation":[{"name":"Provincial Clinical College of Fujian Medical University Fuzhou China"},{"name":"Department of Geriatric Medicine Fujian Provincial Hospital Fuzhou China"},{"name":"Fujian Provincial Center for Geriatrics Fujian Provincial Hospital Fuzhou 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