{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:57Z","timestamp":1762100637206},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":6044,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our previous work on protein kinase C (PKC) and colon cancer has shown altered levels of PKC activity in human colon tumors, as well as activation of PKC by colon tumor promoters such as bile acids. To understand further the role of PKC in colon carcinogenesis, we analyzed the expression of phorbin, a gene induced by PKC activation, in a series of different stages of human colon tumors. As shown by northern blot analyses of poly (A)+ RNA, higher levels of phorbin RNA were seen in 26 colon tumor samples than in their adjacent normal colonic mucosa. There also appeared to be a correlation between the abundance of phorbin RNA in the tumors and the extent of invasion (tumor\u2010to\u2010normal tissue phorbin RNA ratio = 4.2, 8.0, and 11.9 for Dukes' A, B, and C, respectively). Phorbin RNA was also abundant in a human colon cancer line (HT29). We also examined the expression of other mitogen\u2010responsive genes (c\u2010<jats:italic>myc<\/jats:italic>, ODC, and \u03b2\u2010actin) in a set of 19 colon tumor samples. All tumors displayed significant (mean 3.8\u2010fold) increases in the level of c\u2010<jats:italic>myc<\/jats:italic> RNA compared with their adjacent normal colonic mucosa. About 47% and 16% of these tumor samples also showed increased levels of ODC (mean 3.1\u2010fold) and \u03b2\u2010actin (mean 1.6\u2010fold) RNA, respectively. The increased levels of c\u2010<jats:italic>myc<\/jats:italic>, ODC, and \u03b2\u2010actin RNA did not correlate with the extent of tumor invasion. Taken together, these results demonstrate that human colon tumors usually display increased levels of both phorbin and c\u2010<jats:italic>myc<\/jats:italic> RNAs. The marked increases in phorbin RNA suggest that this could serve as a useful biomarker in studies on human colon cancer.<\/jats:p>","DOI":"10.1002\/mc.2940030204","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T20:00:27Z","timestamp":1172088027000},"page":"68-74","source":"Crossref","is-referenced-by-count":31,"title":["Increased levels of phorbin, c\u2010<i>myc<\/i>, and ornithine decarboxylase RNAs in human colon cancer"],"prefix":"10.1002","volume":"3","author":[{"given":"Jose G.","family":"Guillem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam F.","family":"Levy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling Ling","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark D.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Logerfo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth A.","family":"Forde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. Bernard","family":"Weinstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02553649"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197903)43:3<961::AID-CNCR2820430327>3.0.CO;2-W"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240330308"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2821"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/8.2.217"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1987.01400240123023"},{"key":"e_1_2_1_8_2","first-page":"2036","article-title":"Altered levels of protein kinase C and Ca2+\u2010dependent protein kinases in human colon carcinomas","volume":"47","author":"Guillem JG","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0011-3840(87)90002-5"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/318066a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/315768a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700500314"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910410221"},{"key":"e_1_2_1_14_2","first-page":"3964","article-title":"Changes in expression of oncogenes and endogenous retrovirallike sequences induced by AOM","volume":"48","author":"Guillem JG","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.21.6497"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.6.1673"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90238-X"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.243.5.C212"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1298"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1182\/blood.V71.6.1720.1720","article-title":"K562 cells produce and respond to human erythroid\u2010potentiating activity","volume":"71","author":"Avalos BR","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.5.8.1969"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6538699"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19870401)59:7<1289::AID-CNCR2820590710>3.0.CO;2-O"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(87)91074-2"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1985.237"},{"key":"e_1_2_1_26_2","first-page":"3226","article-title":"Ornithine decarboxylase activity in the rat and human colon","volume":"44","author":"Rozhin J","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-198607000-00013"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198407123110202"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/327293a0"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/327298a0"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmc.2940030204","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.2940030204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T16:03:27Z","timestamp":1697990607000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.2940030204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/mc.2940030204"],"URL":"https:\/\/doi.org\/10.1002\/mc.2940030204","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"value":"0899-1987","type":"print"},{"value":"1098-2744","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}