{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:49:18Z","timestamp":1790938158650,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":5679,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Point mutations of c\u2010<jats:italic>ras<\/jats:italic> genes were investigated in human angiosarcomas of the liver associated with occupational exposure to vinyl chloride. DNA prepared from either frozen or paraffin\u2010embedded tissues was amplified by the polymerase chain reaction, and putative point mutations at codons 12, 13, and 61 of c\u2010Ha\u2010<jats:italic>ras<\/jats:italic>, c\u2010Ki\u2010<jats:italic>ras<\/jats:italic>, and N\u2010<jats:italic>ras<\/jats:italic> were analyzed by dot\u2010blot hybridization with allele\u2010specific oligonucleotides. A G\u00b7C\u2192A\u00b7T transition in the second nucleotide at codon 13 of the c\u2010Ki\u2010<jats:italic>ras<\/jats:italic>\u20102 gene was detected in 5 of 6 tumors. This mutation is likely a consequence of vinyl chloride\u2010DNA adduct formation. It leads to the substitution of glycine by aspartic acid in the resulting p21 protein, a consistent amino acid substitution found so far in all types of human cancer exhibiting a codon 13\u2010mutated Ki\u2010<jats:italic>ras<\/jats:italic> gene.<\/jats:p>","DOI":"10.1002\/mc.2940040607","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T20:15:23Z","timestamp":1172088923000},"page":"450-454","source":"Crossref","is-referenced-by-count":70,"title":["Activation of Ki\u2010<i>ras<\/i> gene by point mutation in human liver angiosarcoma associated with vinyl chloride exposure"],"prefix":"10.1002","volume":"4","author":[{"given":"Marie\u2010Jeanne","family":"Marion","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Froment","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Tr\u00e9po","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0013-9351(74)90040-1"},{"key":"e_1_2_1_3_2","first-page":"150","article-title":"Angiosarcoma of the liver in the manufacture of polyvinyl chloride","volume":"16","author":"Creech JL","year":"1974","journal-title":"J Occup Med"},{"key":"e_1_2_1_4_2","volume-title":"Angiosarcoma Registry","author":"Bennett B","year":"1990"},{"key":"e_1_2_1_5_2","first-page":"258","article-title":"On the possible 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