{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:40:29Z","timestamp":1788244829165,"version":"build-2803163510"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":5313,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The neurofibromatosis type 1 (<jats:italic>NF<\/jats:italic>1) gene encodes a 360\u2010residue region showing significant homology to the catalytic domains of both mammalian GTPase\u2010activating protein (GAP) and yeast IRA protein. The product of the GAP\u2010related domain of the <jats:italic>NF<\/jats:italic>1 gene (<jats:italic>NF<\/jats:italic>1\u2010GRD) has been shown to stimulate ras GTPase and consequently to inactivate ras protein. We previously reported that the <jats:italic>NF<\/jats:italic>1\u2010GRD has two types of transcripts, type I and type II, which are generated by an alternative splicing mechanism, and that the differential splicing of the <jats:italic>NF<\/jats:italic>1\u2010GRD may be related to differentiation of neuroectodermal cells. Here we examined the differential expression of type I and type II transcripts of <jats:italic>NF<\/jats:italic>1\u2010GRD in clinical samples of supratentorial malignant brain tumors by the RNA\u2010polymerase chain reaction (PCR) method using frozen tissue sections. Our observations revealed that normal cerebrum predominantly expressed the type II <jats:italic>NF<\/jats:italic>1\u2010GRD transcript, whereas primitive neuroectodermal tumors predominantly expressed the type I transcript. Additionally, although the type I\/type II ratio in astrocytomas varied widely among tissue samples, all glioblastomas showed higher type I\/type II ratios than adjacent brain samples. The RNA\u2010PCR analysis using frozen tissue sections is a useful and sensitive method for detecting genetic markers in clinical tissue samples. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.2940060203","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T20:04:01Z","timestamp":1172088241000},"page":"83-87","source":"Crossref","is-referenced-by-count":18,"title":["Alternative splicing of neurofibromatosis type 1 gene transcript in malignant brain tumors: PCR analysis of frozen\u2010section mRNA"],"prefix":"10.1002","volume":"6","author":[{"given":"Hiroshi","family":"Mochizuki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Nishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet M.","family":"Bruner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polly S. Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor A.","family":"Levin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Saya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2134734"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90253-B"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90024-9"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90149-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90150-D"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90151-4"},{"key":"e_1_2_1_8_2","first-page":"1555","article-title":"Differential expression of two types of the neurofibromatosis type 1 (NF1) gene transcripts related to neuronal differentiation","volume":"6","author":"Nishi T","year":"1991","journal-title":"Oncogene"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00554792"},{"key":"e_1_2_1_10_2","first-page":"149","article-title":"Differential splicing of the neurofibromatosis type 1 gene in the rat: Splice variants homologous with the human are expressed in rat cells","volume":"1","author":"Kyritsis AP","year":"1992","journal-title":"International Journal of Oncology"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/327293a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)92029-J"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmc.2940060203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.2940060203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T11:59:13Z","timestamp":1698062353000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.2940060203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1002\/mc.2940060203"],"URL":"https:\/\/doi.org\/10.1002\/mc.2940060203","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"value":"0899-1987","type":"print"},{"value":"1098-2744","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}