{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:48:53Z","timestamp":1717030133266},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":5313,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A number of primary human breast carcinomas exhibit amplification of the chromosome 11 region containing the <jats:italic>int\u20102\/fgf\u20103<\/jats:italic> proto\u2010oncogene, and progression of breast cancer has been correlated with <jats:italic>int\u20102<\/jats:italic> amplification or with certain restriction fragment length polymorphisms (RFLPs) of the <jats:italic>int\u20102<\/jats:italic> gene. Using the polymerase chain reaction (PCR), we obtained the <jats:italic>int\u20102<\/jats:italic> coding sequences from six primary tumors, four of which exhibited amplification of the <jats:italic>int\u20102<\/jats:italic> gene and one of which exhibited amplification of the neu gene. The majority of these tumors (five of six) were aggressive, as judged by their early recurrence, metastasis, or both. Nucleotide sequencing of PCR products revealed that previously described BamHl and Pstl RFLPs of the int\u20102 gene, as well as a new polymorphism at position 9154, were located within the intron between the second and third exons. A seventh tumor was used to localize one of the Pstl RFLPs 5 bp from the splice\u2010acceptor site of the third exon. However, none of the tumor DNAs analyzed showed differences in the int\u20102 protein coding regions when compared with normal placenta DNA. These results imply that aggressive human breast cancers encode an unaltered form of the int\u20102 protein. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.2940060405","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T20:44:17Z","timestamp":1172090657000},"page":"243-251","source":"Crossref","is-referenced-by-count":5,"title":["Sequence analysis of the <i>int\u20102\/fgf\u20103<\/i> gene in aggressive human breast carcinomas"],"prefix":"10.1002","volume":"6","author":[{"given":"Shari L.","family":"Meyers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaquelin 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