{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:18:16Z","timestamp":1718651896097},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":3914,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cellular differentiation and proliferation are dependent upon phosphorylation by endogenous protein kinase C (a) isozymes in many cell types. Western blotting with a C\u2010terminally directed rabbit polyclonal anti\u2010PKC \u03b6 antibody detected a doublet of approximately 81 kDa in normal hamster pancreatic tissue and hamster pancreatic carcinoma (PC\u20101) and human pancreatic carcinoma (PANC\u20101) cells, Preabsorption of the antibody with the specific peptide blocked the appearance of the 81\u2013kDa band, indicating that the band was specifically recognized by the PKC \u03b6 antibody. In contrast, antibodies for PKC \u03b1, \u03b2, \u03b3, \u03b4, and \u03f5 failed to show specific immunoreactivity for normal pancreatic tissue or PANC\u20101 or PC\u20101 cells. Immunocytochemical analysis identified PKC \u03b6 in the cytoplasm of ductules and large ducts, to a lesser extent in the islets of the hamster pancreas, and in the normal cultured pancreatic duct epithelial cells and pancreatic carcinoma (PANC\u20101 and PC\u20101) cell lines. Specific reactivity was seen by electron microscopy in the ductal cells of the normal pancreatic tissue. In normal pancreatic ductal tissue and primary pancreatic ductal hyperplasia and carcinoma, the proportional labeling of PKC \u03b6 in nuclei and cytoplasm was similar. Our results demonstrating the presence of PKC \u03b6 isozyme in the normal pancreas, cultured normal pancreatic duct epithelial cells, and pancreatic carcinoma cells or carcinoma tissue suggests a role for this isozyme in the normal physiology of the pancreas and perhaps in pancreatic carcinoma. \u00a9 1995 Wiley\u2010 Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.2940140310","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T21:11:50Z","timestamp":1172092310000},"page":"205-213","source":"Crossref","is-referenced-by-count":3,"title":["Identification of protein kinase C \u03b6 isozyme in hamster pancreas and pancreatic carcinoma cell lines"],"prefix":"10.1002","volume":"14","author":[{"given":"Satish C.","family":"Nair","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parvis 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