{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:57:31Z","timestamp":1792267051002,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer and the deadliest liver disease. It is imperative to understand the underlying molecular mechanisms involved in the development of HCC. Monocarboxylate transporter\u20101 (MCT1) is a proton\u2010coupled protein that facilitates the bidirectional transport of monocarboxylates, such as lactate and pyruvate, across the plasma membrane to maintain the cellular metabolism and energy supply. MCT1 was found to be upregulated in human HCC specimens, and its inhibition reduced xenograft tumor growth. However, the role of MCT1 in HCC remains to be further investigated using immune\u2010competent in vivo models. To better understand the role of MCT1 in HCC, we established liver\u2010specific MCT1 knockout mice. We found that deletion of MCT1 in liver cells did not affect morphology, proliferation, or apoptosis. DEN\/CCl4 model, where a single injection of DEN is followed by repeated injections of CCl4, was used to induce HCC in mice. Intriguingly, we found that liver\u2010specific knockout of MCT1 was not sufficient to reduce the size or count of DEN\/CCl4\u2010induced liver tumors. In addition, we used immunohistochemical staining to evaluate the expression of Ki67, collagen A1, and myeloperoxidase, and we found that MCT1 knockout was not able to hinder the proliferation, fibrosis, and inflammation in the DEN\/CCl4\u2010induced HCC tumors. In conclusion, MCT1 is dispensable for HCC development, and its deletion was insufficient to alleviate the phenotypic repercussions of HCC tumors in the DEN\/CCl4\u2010induced HCC model.<\/jats:p>","DOI":"10.1002\/mc.70021","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T17:53:58Z","timestamp":1753984438000},"page":"1724-1734","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Monocarboxylate Transporter\u20101 Is Dispensable for Hepatocellular Carcinoma Development"],"prefix":"10.1002","volume":"64","author":[{"given":"Shaimaa A.","family":"Gad","sequence":"first","affiliation":[{"name":"Departments of Surgery, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"},{"name":"Departments of Cancer Biology, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"},{"name":"Department of Pharmacology, Medical Research and Clinical Studies Institute National Research Center  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3396-2228","authenticated-orcid":false,"given":"Bryan","family":"Bridgeman","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"},{"name":"Departments of Cancer Biology, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle","family":"Boedeker","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"},{"name":"Departments of Cancer Biology, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzhong","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8093-3094","authenticated-orcid":false,"given":"Wei","family":"Qiu","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois USA"},{"name":"Departments of Cancer Biology, Pathology Loyola University Chicago Stritch School of Medicine Maywood Illinois 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Lactate\u2010Fueled Respiration Selectively Kills Hypoxic Tumor Cells in Mice","volume":"118","author":"Sonveaux P.","year":"2008","journal-title":"Journal of Clinical Investigation"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1610466"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504017X14902648894463"},{"key":"e_1_2_10_18_1","first-page":"4901","article-title":"Monocarboxylate Transporter 1 Blockade With AZD3965 Inhibits Colorectal Tumor Growth and Modulates the Tumor Immune Microenvironment","volume":"80","author":"Beloueche\u2010Babari M.","year":"2020","journal-title":"Cancer 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