{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:36:04Z","timestamp":1792503364340,"version":"4.5.0"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tertiary lymphoid structures (TLS) demonstrate prognostic significance and associations with immunotherapy response in gastrointestinal malignancies, though their regulatory mechanisms remain incompletely defined. The current understanding of TLS at single\u2010cell resolution is limited. Here, we integrated single\u2010cell and spatial transcriptomics with TLS\u2010specific signatures to map spatial distributions and chemokine signaling within colorectal (CRC) and gastric cancer (GC) microenvironments. We identified significant enrichment of characteristic T cell and macrophage subsets in the TLS regions. Subpopulation analyses revealed distinct cellular interaction networks: CRC exhibited robust intercellular communication among effector CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, exhausted CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, tissue\u2010resident CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, CD16\n                    <jats:sup>+<\/jats:sup>\n                    monocyte\u2010derived macrophages, C1QC\n                    <jats:sup>+<\/jats:sup>\n                    macrophages, and SPP1\n                    <jats:sup>+<\/jats:sup>\n                    macrophages. Conversely, GC featured pronounced interactions between interferon\u2010stimulated gene\u2010positive (ISG\n                    <jats:sup>+<\/jats:sup>\n                    ) CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and ISG15\n                    <jats:sup>+<\/jats:sup>\n                    macrophages. Further analyses suggest CD16\n                    <jats:sup>+<\/jats:sup>\n                    monocyte\u2010derived macrophages may recruit effector CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells via the CXCL16\u2010CXCR6 ligand\u2010receptor pair in CRC, while ISG15\n                    <jats:sup>+<\/jats:sup>\n                    macrophages may utilize dual CXCL16\u2010CXCR6 and CXCL10\u2010CXCR3 pairs to recruit ISG\n                    <jats:sup>+<\/jats:sup>\n                    effector CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in GC. Our study uncovers spatially resolved, cancer\u2010type\u2010specific immune recruitment circuits within TLS, providing mechanistic insights into their functional organization and potential therapeutic targeting.\n                  <\/jats:p>","DOI":"10.1002\/mc.70058","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T21:05:17Z","timestamp":1763499917000},"page":"153-166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Single\u2010Cell and Spatial Transcriptomic Analysis Reveals Shared and Cancer\u2010Type\u2010Specific Cellular Interactions and Chemokine Signaling Associated With Tertiary Lymphoid Structures in Colorectal and Gastric Cancers"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-5409","authenticated-orcid":false,"given":"Chuyi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology Soochow University Medical School Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenshu","family":"Chen","sequence":"additional","affiliation":[{"name":"The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, Department of Pathology and Institute of Molecular Pathology The First Affiliated Hospital, Jiangxi Medical College, Nanchang University Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Children's Hospital of Soochow University, Soochow University Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhua","family":"Su","sequence":"additional","affiliation":[{"name":"Suzhou Institute of Systems Medicine Chinese Academy of Medical Sciences &amp; Peking Union Medical College Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pathology Soochow University Medical School Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Soochow University Medical School Suzhou China"},{"name":"The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, Department of Pathology and Institute of Molecular Pathology The First Affiliated Hospital, Jiangxi Medical College, Nanchang University Nanchang China"},{"name":"Department of Pathology Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.03.021"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05680-3"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.12.028"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06199-x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-019-0144-6"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-025-03889-3"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2025.101934"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-024-00579-w"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-025-03318-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2025.217491"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-52153-4"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-025-03635-y"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2025.03.011"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08426-5"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.ado8395"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59341-w"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02396-7"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.91487"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1221532"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.09.005"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08466-x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mog2.89"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.04.015"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39592-1"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2637"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-025-02084-z"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00940-2"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mog2.21"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adg0539"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3862"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-022-00627-9"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105154"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mog2.92"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29753-z"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxp112"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abg7836"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers6020969"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00771.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-025-03889-3"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.007"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00765"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058-017-0864-z"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32982"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-021-01635-2"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15030828"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2024-009231"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1158\/2767-9764.CRC-24-0534"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-023-11738-w"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.32604\/or.2025.058957"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00574-3"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389-025-00549-2"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14521"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074-7613(01)00226-6"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.8.4880"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100102"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.70058","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mc.70058","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.70058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T14:48:46Z","timestamp":1770389326000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.70058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,18]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/mc.70058"],"URL":"https:\/\/doi.org\/10.1002\/mc.70058","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"value":"0899-1987","type":"print"},{"value":"1098-2744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,18]]},"assertion":[{"value":"2025-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}