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This study aims to investigate the functional role of long non\u2010coding RNA (lncRNA) PTPRG antisense RNA 1 (PTPRG\u2010AS1) in NSCLC progression using in vitro models, focusing on its potential as a regulator of key oncogenic processes and its interaction with the IGF\u20101\/PI3K\/AKT signaling pathway to identify novel therapeutic targets. PTPRG\u2010AS1 expression in NSCLC cell lines (A549, H1299, NCI\u2010H226) and normal lung cells (Beas\u20102B) was analyzed using RT\u2010qPCR. PTPRG\u2010AS1 was silenced with siRNA, and its effects on cell proliferation, migration, invasion, colony formation, apoptosis, and angiogenesis\u2010related factors were evaluated. Western blot analysis assessed components of the PI3K\/AKT pathway. LncRNA PTPRG\u2010AS1 was significantly upregulated in NSCLC cells, particularly in H1299. Our results showed that PTPRG\u2010AS1 knockdown inhibited cell proliferation, migration, and invasion, while promoting cell apoptosis. IGF\u20101 treatment reversed these effects. PTPRG\u2010AS1 knockdown reduced levels of angiogenesis\u2010related factors, including VEGF and bFGF. Additionally, silencing PTPRG\u2010AS1 decreased expression of key PI3K\/AKT pathway components, while exogenous IGF\u20101 reactivated this signaling cascade. PTPRG\u2010AS1 plays a critical role in NSCLC progression by modulating the IGF\u20101\/PI3K\/AKT signaling pathway. Targeting PTPRG\u2010AS1 offers a promising therapeutic strategy for NSCLC, warranting further investigation into its clinical implications.<\/jats:p>","DOI":"10.1002\/mc.70073","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T19:09:18Z","timestamp":1774897758000},"page":"728-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long Non\u2010Coding RNA PTPRG\u2010AS1 Promotes Proliferation, Migration, and Invasion of Non\u2010Small Cell Lung Cancer Cells via Modulation of the IGF1\/PI3K\/AKT Signaling Pathway"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4916-2138","authenticated-orcid":false,"given":"Gongzhang","family":"Su","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhisheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingcai","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Shandong First Medical University Jinan Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_13_3_1","first-page":"87","volume-title":"Non\u2010small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). 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