{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:54:05Z","timestamp":1773212045088,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172874"],"award-info":[{"award-number":["82172874"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cyclin L1 (CCNL1) is highly expressed in multiple cancer types and has been linked to poor prognosis. However, the expression pattern of CCNL1 in breast cancer and its specific role in regulating breast cancer progression remain largely unknown. This study used cell and molecular biology techniques to examine how CCNL1 regulates the proliferation, invasion, migration, and epithelial\u2010mesenchymal transition (EMT) of breast cancer cells. The applied methods encompassed plasmid transfection, Transwell assay, wound\u2010healing assay, Western blot analysis, co\u2010immunoprecipitation (Co\u2010IP), and rescue assay. For the analysis of CCNL1\u2010related factors and pathways, bioinformatics platforms including Metascape and HURI were also employed. CCNL1 is highly expressed in breast cancer cells and is associated with a poor prognosis. CCNL1 overexpression increased breast cancer cell invasion and migration and accelerated proliferation. Overexpression of CCNL1 was found to upregulate the mesenchymal marker Vimentin and downregulate the epithelial marker E\u2010cadherin expression. There is close relationship between CCNL1, the NF\u2010\u03baB and PI3K\/AKT signaling pathways. The direct interaction is verified between CCNL1 and DVL3 by Co\u2010IP, indicating a negative correlation between the two proteins. CCNL1 overexpression affects breast cancer cells' paclitaxel sensitivity through the PI3K\/AKT pathway. CCNL1 activates the NF\u2010\u03baB signaling pathway through its interaction with DVL3; additionally, it promotes the PI3K\/AKT pathway. Together, these two mechanisms enable CCNL1 to exert a regulatory role in the progression of breast cancer.<\/jats:p>","DOI":"10.1002\/mc.70090","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T20:40:47Z","timestamp":1770151247000},"page":"448-464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CCNL1 Activates the NF\u2010\u03baB Pathway Through DVL3 Inhibition and PI3K\/AKT Pathway Promotion in Breast Cancer"],"prefix":"10.1002","volume":"65","author":[{"given":"Dan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Runfen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology Cancer Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyuan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunsheng","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiulan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baocun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"},{"name":"Department of Pathology Cancer Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4522-545X","authenticated-orcid":false,"given":"Tieju","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology Tianjin Medical University Tianjin China"},{"name":"Department of Pathology General Hospital of Tianjin Medical University Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.08.012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35021093"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050518"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708188200"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602400"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20671"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-022-00326-8"},{"key":"e_1_2_9_10_1","first-page":"1259","article-title":"The Effect of the KOZAK Sequence on the Expression of the CCNL1 Gene in Breast Cancer MDA\u2010MB\u2010231 Cells. Modern Oncology","volume":"07","author":"Peng L.","year":"2011","journal-title":"Medicine"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.05.021"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12851"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.16169"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-024-02140-9"},{"issue":"1","key":"e_1_2_9_15_1","first-page":"E112","article-title":"Dishevelled\u20103 Activates p65 to Upregulate p120\u2010Catenin Transcription via a p38\u2010dependent Pathway in Non\u2010Small Cell Lung Cancer","volume":"54","author":"Zhao H.","year":"2015","journal-title":"Molecular Carcinogenesis"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202255044"},{"issue":"06","key":"e_1_2_9_17_1","first-page":"771","article-title":"Research on the Mechanism of Bushen Huoxue Formula (a Traditional Chinese Medicine Formula for Nourishing Kidney and Promoting Blood Circulation) in Treating Endometriosis Based on the PI3K\/Akt\/NF\u2010\u03baB Pathway","volume":"39","author":"Zhonglin M.","year":"2022","journal-title":"Tianjin University of Traditional Chinese Medicine"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/4754195","article-title":"Sulforaphene Ameliorates Neuroinflammation and Hyperphosphorylated Tau Protein via Regulating the PI3K\/Akt\/GSK\u20103\u03b2 Pathway in Experimental Models of Alzheimer's Disease","volume":"2020","author":"Yang W.","year":"2020","journal-title":"Oxidative Medicine and Cellular Longevity"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2022-0070-RA"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5398.83"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00486-7"},{"issue":"10","key":"e_1_2_9_22_1","first-page":"4122","article-title":"A Simple Specific Pattern of Chromosomal Aberrations at Early Stages of Head and Neck Squamous Cell Carcinomas: PIK3CA but not p63 Gene as a Likely Target of 3q26\u2010qter Gains","volume":"61","author":"Redon R.","year":"2001","journal-title":"Cancer Research"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2022.113375"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5553"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093285"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1030-2"},{"issue":"01","key":"e_1_2_9_27_1","first-page":"12","article-title":"The Effects of Long\u2010Term Low\u2010Dose Arsenic Exposure on PKB\/Akt and Its Downstream Signaling Factors IKK","volume":"29","author":"Shiyi L.","year":"2014","journal-title":"I\u03baB, and NF\u2010\u03baB in HaCat Cells. 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