{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T13:21:42Z","timestamp":1775827302907,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["56\/7\/2019_Hae\u2010BMS"],"award-info":[{"award-number":["56\/7\/2019_Hae\u2010BMS"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Early diagnosis of acute promyelocytic leukemia (APL), driven by PML\u2010RARA oncoprotein, is vital for survival, as delays can cause fatal coagulopathy without prompt therapeutic intervention of all\u2010trans retinoic acid and arsenic trioxide. Although APL is diagnosed using microscopy, immunophenotyping, and FISH\/PCR for PML\u2010RARA, morphological overlap with acute myeloid leukemia (AML) \u2010M5 and AML\u2010M2 complicates identification. In resource\u2010limited settings, unavailability of real time \u2010quantitiative PCR (RQ\u2010PCR) or delays in FISH\/qualitative RT\u2010PCR results increase the risk of missed or late diagnosis with fatal outcomes. Podoplanin (PDPN), a glycoprotein overexpressed in APL cells, interacts with platelets to mediate thrombosis. We evaluated PDPN expression, regulation, and diagnostic potential in APL. PDPN mRNA (RQ\u2010PCR) and surface protein (flow cytometry) were significantly higher in APL than non\u2010APL AML (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001), consistent with TCGA\u2010LAML and BEATAML1.0 datasets. Sensitivity and specificity were 80.7% and 71.43% by RQ\u2010PCR, and 92.86% and 100% by flow cytometry, respectively. Chromatin immunoprecipitation followed by quantitative PCR (ChIP\u2010qPCR) and TGF\u2010 \u03b21 stimulation confirmed SMAD binding and PDPN upregulation. Pharmacological inhibition of TGF\u2010\u03b21 ligand using luspatercept reduced SMAD phosphorylation and PDPN expression, indicating TGF\u2010\u03b2\/SMAD transcriptionally regulates PDPN. Additionally, ELISA\u2010based serum profiling showed significantly elevated TGF\u2010\u03b21 levels in APL patients compared to non\u2010APL AML (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001). These findings identify PDPN overexpression as a downstream consequence of TGF\u2010\u03b2\/SMAD signaling and highlight its potential as a diagnostic biomarker for APL.\n                  <\/jats:p>","DOI":"10.1002\/mc.70096","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:52:21Z","timestamp":1770965541000},"page":"556-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TGF\u03b2\u2010Mediated Overexpression of Podoplanin Serves as a Potential Diagnostic Biomarker in Acute Promyelocytic Leukemia"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9356-8445","authenticated-orcid":false,"given":"Akash","family":"Maity","sequence":"first","affiliation":[{"name":"Hasan Laboratory, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre Navi Mumbai Maharashtra India"},{"name":"Homi Bhabha National Institute (HBNI), Anushaktinagar Mumbai Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Hasan Laboratory, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre Navi Mumbai Maharashtra India"},{"name":"S.V.B. 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