{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T14:08:51Z","timestamp":1778854131721,"version":"3.51.4"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Prostate cancer (PCa) is the second most frequently diagnosed malignancy among men and the sixth leading cause of cancer\u2010related mortality worldwide. Radical prostatectomy remains the standard treatment for localized disease; however, approximately one\u2010third of patients develop biochemical recurrence within 10 years. Although prostate\u2010specific antigen (PSA) is the primary biomarker used for diagnosis and monitoring, additional molecular markers such as\n                    <jats:italic>PTEN<\/jats:italic>\n                    loss and\n                    <jats:italic>MYC<\/jats:italic>\n                    amplification have been investigated to improve prognostic stratification. Liquid biopsy has emerged as a promising tool in oncology, yet the clinical significance of circulating tumor cells (CTCs) in early\u2010stage PCa remains uncertain. This prospective, single\u2010center study included 50 patients with localized prostate cancer from the Brazilian Amazon region who underwent radical prostatectomy in 2023. Peripheral blood samples were collected preoperatively and postoperatively for CTC enumeration and molecular characterization using Cytelligen SET\u2010iFISH and the CellSearch system.\n                    <jats:italic>PTEN<\/jats:italic>\n                    and\n                    <jats:italic>MYC<\/jats:italic>\n                    status were evaluated to further characterize the detected CTC population. CTCs were detected in all patients prior to surgery, with a significant reduction observed after radical prostatectomy (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001); 88% of patients had no detectable CTCs postoperatively.\n                    <jats:italic>PTEN<\/jats:italic>\n                    loss was identified in 12% of patients, all of whom presented with ISUP grade \u2265 2 disease.\n                    <jats:italic>MYC<\/jats:italic>\n                    amplification was rare, observed in only one patient with Gleason score 7 and concomitant\n                    <jats:italic>PTEN<\/jats:italic>\n                    deletion. These findings suggest that perioperative CTC detection may provide clinically relevant prognostic information in localized PCa.\n                    <jats:italic>PTEN<\/jats:italic>\n                    loss appears to represent an early molecular alteration associated with adverse pathological features. Longer follow\u2010up is needed to determine the predictive value of these biomarkers for recurrence and long\u2010term outcomes.\n                  <\/jats:p>","DOI":"10.1002\/mc.70104","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:36:48Z","timestamp":1772966208000},"page":"665-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Profiling of Circulating Tumor Cells and\n                    <i>MYC\/PTEN<\/i>\n                    Alterations in Prostate Cancer Patients Undergoing Radical Prostatectomy in Amazon Population"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2557-011X","authenticated-orcid":false,"given":"Juliana Ramos","family":"Chaves","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Medicina Molecular Universidade Federal do Par\u00e1 Bel\u00e9m Par\u00e1 Brazil"},{"name":"Servi\u00e7o de Urologia 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Souza","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Urologia Hospital Ophir Loyola Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Janete Nahum","family":"Gomes","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Urologia Hospital Ophir Loyola Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio Augusto Antunes","family":"Ramos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Medicina Molecular Universidade Federal do Par\u00e1 Bel\u00e9m Par\u00e1 Brazil"},{"name":"Servi\u00e7o de Urologia Hospital Ophir Loyola Bel\u00e9m Par\u00e1 Brazil"},{"name":"N\u00facleo de Pesquisas em Oncologia, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto R. dos Mundurucus, 4487 \u2010 Guam\u00e1 Bel\u00e9m Par\u00e1 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