{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T14:08:58Z","timestamp":1778854138128,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Osteosarcoma (OS) [1] is the most prevalent primary bone cancer and is highly prone to both local invasion and metastasis. FAM162A, a protein implicated in cell growth transformation, is recognized as an oncogene in various cancers. Its role in osteosarcoma, however, remains poorly understood. This study investigates the role of FAM162A in the OS development. We identified that FAM162A was observably upregulated in osteosarcoma and inversely correlated with the prognosis of patients using bioinformatics and immunohistochemical analysis. The CCK\u20108, colony formation, scratch, and trans\u2010well migration assays were performed to detect the effects of FAM162A on OS cell proliferation and migration. Then we found FAM162A\u2010associated pathways in OS using GO and KEGG analyses and verified and used the downstream factor HIF\u20101\u03b1 inhibitor to restore the OS cell proliferation induced by FAM162A overexpression to detect the mechanism of FAM162A in OS. Mechanistically, FAM162A facilitates glycolysis by activating HIF\u20101\u03b1 and subsequently PKM2, a critical glycolytic gene, thereby advancing OS progression. These insights elucidated FAM162A's involvement in OS and its impact on glucose metabolism reprogramming, underscoring its potential as a therapeutic target.<\/jats:p>","DOI":"10.1002\/mc.70112","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:37:57Z","timestamp":1774546677000},"page":"715-727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["FAM162A Promotes Osteosarcoma Progression Via Activating HIF\u20101\u03b1 to Enhance Glycolysis"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6170-1963","authenticated-orcid":false,"given":"Jun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Emergency Surgery Department, Sinopharm Dongfeng General Hospital Hubei University of Medicine Shiyan Hubei China"},{"name":"Emergency Surgery Department, Taikang Clinical College of Wuhan University Taikang Tongji Wuhan Hospital Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pain and Rehabilitation, Wuhan Clinical Research Center for Acupotomy Medicine Wuhan Third Hospital Wuhan Hubei China"},{"name":"Department of Medicine, School of Medicine Wuhan University of Science and Technology Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Fan","sequence":"additional","affiliation":[{"name":"Cancer Center, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubo","family":"Cao","sequence":"additional","affiliation":[{"name":"Cancer Center, Union Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuang","family":"Miao","sequence":"additional","affiliation":[{"name":"Emergency Surgery Department, Sinopharm Dongfeng General Hospital Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenxin","family":"Guo","sequence":"additional","affiliation":[{"name":"Emergency Surgery Department, Sinopharm Dongfeng General Hospital Hubei University of Medicine Shiyan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningning","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine Wuhan University of Science and Technology Wuhan Hubei China"},{"name":"Graduate Training Base of Wuhan Third Hospital Wuhan University of Science and Technology Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haotian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine Wuhan University of Science and Technology Wuhan Hubei China"},{"name":"Graduate Training Base of Wuhan Third Hospital Wuhan University of Science and Technology Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqian","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605211"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2019.100221"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2020.12.024"},{"key":"e_1_2_10_5_1","volume-title":"Pathology and Genetics of Tumours of Soft Tissue and Bone, vol. 4","author":"Fletcher C. 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