{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T18:56:39Z","timestamp":1781636199631,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) exhibits considerable molecular heterogeneity. This study aimed to delineate the mutational landscape and investigate the associations between frequently mutated genes and key clinicopathological features in a single\u2010center cohort of CRC patients using next\u2010generation sequencing (NGS). This study included 381 patients with pathologically confirmed colorectal cancer. Tumor tissue samples were collected and subjected to targeted sequencing and variant analysis of 40 cancer\u2010related genes using an NGS platform. Associations between gene mutation status and clinicopathological features\u2014including tumor location, clinical stage, and MSI status\u2014were assessed using the\n                    <jats:italic>\u03c7<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    test. Sequencing analysis revealed that 12 patients harbored no detectable mutations in the targeted genes. Among the remaining 369 patients, somatic variants were identified across 30 genes. Regarding mutational patterns, 115 cases (30.2%) exhibited single\u2010gene mutations, 158 cases (41.5%) showed two co\u2010occurring mutations, and 96 cases (25.2%) carried alterations in three or more genes. The most frequently mutated genes were TP53 (76.9%), KRAS (47.8%), and PIK3CA (18.9%). TP53 mutations were significantly enriched in left\u2010sided colon cancers (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). In contrast, both KRAS (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.010) and PIK3CA (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.001) mutations were significantly associated with right\u2010sided colon cancers. Furthermore, the frequency of PIK3CA mutations was significantly higher in MSI\u2010high tumors compared to MSS tumors. This study demonstrates significant associations between specific gene mutations and distinct clinicopathological characteristics. The findings underscore the importance of integrating molecular profiling with conventional clinicopathological parameters for precise stratification.\n                  <\/jats:p>","DOI":"10.1002\/mc.70123","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:34:21Z","timestamp":1776796461000},"page":"863-873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Next\u2010Generation Sequencing\u2010Based Analysis of the Genetic Mutation Spectrum in Colorectal Cancer: A Large Single\u2011Center Study From Southeast China With Cross\u2011Population Comparison"],"prefix":"10.1002","volume":"65","author":[{"given":"Huijuan","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Pathology, Zhongshan Hospital (Xiamen) Fudan University Xiamen China"},{"name":"Clinical Research Center for Precision Medicine of Abdominal Tumor of Fujian Province Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruichen","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Zhongshan Hospital (Xiamen) Fudan University Xiamen China"},{"name":"Clinical Research Center for Precision Medicine of Abdominal Tumor of Fujian Province Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Research Center for Precision Medicine of Abdominal Tumor of Fujian Province Xiamen China"},{"name":"Department of Blood Transfusion, Zhongshan Hospital (Xiamen) Fudan University Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology, Zhongshan Hospital (Xiamen) Fudan University Xiamen China"},{"name":"Clinical Research Center for Precision Medicine of Abdominal Tumor of Fujian Province Xiamen 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Precision Medicine of Abdominal Tumor of Fujian Province Xiamen 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