{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:30:48Z","timestamp":1786559448297,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Prevention is desirable to reduce suffering and death from prostate cancer. However, a clinical\u2010grade intervention for the prevention of this malignancy is still lacking. This study was undertaken to determine the feasibility of prostate cancer prevention by broccoli constituent sulforaphane (SFN) because epidemiological studies have suggested an inverse association between intake of broccoli and the risk of prostate cancer. Oral administration of 1\u2009mg SFN\/mouse (three times\/week) to 5\u2010week\u2010old male Hi\u2010Myc transgenic mice for 5 weeks decreased the incidence of prostatic adenocarcinoma in situ by about 54% without causing weight loss or any other side effects. Existing literature strongly implicates fatty acid synthesis in prostate cancer progression. Therefore, we determined the effect of SFN treatment on fatty acid synthesis. Prostate cancer prevention by SFN in Hi\u2010Myc mice was accompanied by a decrease in: (a) the uptake of\n                    <jats:sup>11<\/jats:sup>\n                    C\u2010acetate in the prostate of live mice; (b) plasma levels of fatty acid synthesis intermediates acetyl\u2010CoA and malonyl\u2010CoA; (c) circulating levels of total free fatty acids (TFFA); (d) downregulation of fatty acid synthesis enzyme proteins acetyl\u2010CoA carboxylase 1 and fatty acid synthase; and (e) plasma levels of interkeukin\u201010 and interleukin\u201012. Because prostate cancer in Hi\u2010Myc mice is driven by Myc, we determined the effect of overexpression of c\u2010Myc in 22Rv1 cells on TFFA level. Overexpression of c\u2010Myc conferred partial protection against TFFA suppression by SFN treatment. In summary, this study reveals that SFN administration prevents prostate cancer development in Hi\u2010Myc mice by suppressing fatty acid synthesis.\n                  <\/jats:p>","DOI":"10.1002\/mc.70139","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T18:37:40Z","timestamp":1781807860000},"page":"1052-1062","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevention of Myc\u2010Driven Prostate Cancer in Mice by Oral Administration of Sulforaphane"],"prefix":"10.1002","volume":"65","author":[{"given":"Krishna B.","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Ryeong","family":"Hahm","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce L.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Division of Health Services Research, Department of Urology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivendra V.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA"},{"name":"UPMC Hillman Cancer Center University of Pittsburgh Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.70043"},{"issue":"3","key":"e_1_2_11_3_1","first-page":"228","article-title":"The Risk Factors of Prostate Cancer and Its Prevention: A Literature Review","volume":"48","author":"Perdana N. 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