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Clinical trials targeting immune responses post\u2010ICH have encountered setbacks, potentially attributable to the substantial cellular heterogeneity and intricate intercellular networks within the brain. Here, we present a pioneering investigation utilizing single\u2010cell RNA sequencing and spatial transcriptome profiling at hyperacute (1\u00a0h), acute (24\u00a0h), and subacute (7 days) intervals post\u2010ICH, aimed at unraveling the dynamic immunological landscape and spatial distributions within the cerebral tissue. Our comprehensive analysis revealed distinct cell differentiation patterns among myeloid and lymphocyte populations, along with delineated spatial distributions across various brain regions. Notably, we identified a subset of lymphocytes characterized by the expression of Spp1 and Lyz2, termed macrophage\u2010associated lymphocytes, which exhibited close interactions with myeloid cells. Specifically, we observed prominent interactions between Lgmn+Macro\u2010T cells and microglia through the spp1\u2013cd44 pathway during the acute phase post\u2010ICH in the choroid plexus. These findings represent a significant advancement in our understanding of immune cell dynamics at single\u2010cell resolution across distinct post\u2010ICH time points, thereby laying the groundwork for exploring critical temporal windows and informing the development of targeted therapeutic strategies.<\/jats:p>","DOI":"10.1002\/mco2.635","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T03:07:21Z","timestamp":1720580841000},"source":"Crossref","is-referenced-by-count":14,"title":["Unraveling dynamic immunological landscapes in intracerebral hemorrhage: insights from single\u2010cell and spatial transcriptomic profiling"],"prefix":"10.1002","volume":"5","author":[{"given":"Lingui","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 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