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Menstrual blood\u2010derived mesenchymal stem cells (MenSCs) can ameliorate liver fibrosis through paracrine. Single\u2010cell RNA sequencing (scRNA\u2010seq) may be used to explore the roadmap of activated hepatic stellate cell (aHSC) inactivation to target liver fibrosis. This study established HBV transgenic (HBV\u2010Tg) mouse model of carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced liver fibrosis and demonstrated that MenSCs migrated to the injured liver to improve serological indices and reduce fibrotic accumulation. RNA\u2010bulk analysis revealed that MenSCs mediated extracellular matrix accumulation and cell adhesion. Liver parenchymal cells and nonparenchymal cells were identified by scRNA\u2010seq in the control, CCl<jats:sub>4<\/jats:sub>, and MenSC groups, revealing the heterogeneity of fibroblasts\/HSCs. A CellChat analysis revealed that diminished intercellular adhesion molecule (ICAM) signaling is vital for MenSC therapy. Specifically, <jats:italic>Icam1<\/jats:italic> in aHSCs acted on <jats:italic>Itgal<\/jats:italic>\/<jats:italic>Itgb2<\/jats:italic> and <jats:italic>Itgam<\/jats:italic>\/<jats:italic>Itgb2<\/jats:italic> in neutrophils, causing decreased adhesion. The expression of <jats:italic>Itgal<\/jats:italic>, <jats:italic>Itgam<\/jats:italic>, and <jats:italic>Itgb2<\/jats:italic> was higher in CCl<jats:sub>4<\/jats:sub> group than in the control group and decreased after MenSC therapy in neutrophil clusters. The <jats:italic>Lcn2<\/jats:italic>, <jats:italic>Pglyrp1<\/jats:italic>, <jats:italic>Wfdc21<\/jats:italic>, and <jats:italic>Mmp8<\/jats:italic> had high expression and may be potential targets in neutrophils. 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