{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T14:25:44Z","timestamp":1788791144942,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["SGR771and2019FI_B00673"],"award-info":[{"award-number":["SGR771and2019FI_B00673"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["CAN 2017\/1066"],"award-info":[{"award-number":["CAN 2017\/1066"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008316","name":"P. O. Zetterlings stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Colorectal liver metastases grow following different histologic growth patterns (HGPs), classified as desmoplastic and nondesmoplastic (dHGP, non\u2010dHGP), being the latter associated with worst prognosis. This study aimed to investigate the tumor microenvironment (TME) between HGPs supporting different survival. Multiplexed immunohistochemical staining was performed with the Opal7 system in a 100\u2010patients cohort to evaluate the tumor\u2013liver interface with three different cell panels: lymphoid, myeloid, and carcinoma\u2010associated fibroblasts. Differences between HGPs were assessed by Mann\u2013Whitney\n                    <jats:italic>U<\/jats:italic>\n                    test with Pratt correction and Holm\u2013Bonferroni multitest adjustment. Cytotoxic T\u2010cells were more abundant in tumoral areas of dHGP, while non\u2010dHGP had higher macrophages infiltration, Th2, CD163\n                    <jats:sup>+<\/jats:sup>\n                    , and Calprotectin\n                    <jats:sup>+<\/jats:sup>\n                    cells as well as higher pSMAD2 expression. Regarding carcinoma\u2010associated fibroblasts, several subsets expressing COL1A1 were enriched in dHGP, while \u03b1SMA\n                    <jats:sup>low<\/jats:sup>\n                    _single cells were present at higher densities in non\u2010dHGP. Interestingly, Calprotectin\n                    <jats:sup>+<\/jats:sup>\n                    cells confer better prognoses in non\u2010dHGP, identifying a subgroup of good outcome patients that unexpectedly also show an enrichment in other myeloid cells. In summary, our results illustrate different TME landscapes with respect to HGPs. dHGP presents a higher degree of immunocompetence, higher amounts of Collagen 1 as well as lesser presence of myeloid cell populations, features that might be influencing on the better prognosis of encapsulated metastases.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70000","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T08:43:36Z","timestamp":1732005816000},"source":"Crossref","is-referenced-by-count":6,"title":["The histological growth patterns in liver metastases from colorectal cancer display differences in lymphoid, myeloid, and mesenchymal cells"],"prefix":"10.1002","volume":"5","author":[{"given":"Gemma","family":"Garcia\u2010Vici\u00e9n","sequence":"first","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"},{"name":"Department of Pathology Hospital Universitari de Bellvitge L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Micke","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Carlos","family":"Ruffinelli","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"},{"name":"Department of Medical Oncology Institut Catal\u00e0 d'Oncologia, L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristel","family":"Mils","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"},{"name":"Department of Surgery Hospital Universitari de Bellvitge L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Ba\u00f1uls","sequence":"additional","affiliation":[{"name":"Program Against Cancer Therapeutic Resistance (ProCURE) Institut Catal\u00e0 d'Oncologia, L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Molina","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Pardo","sequence":"additional","affiliation":[{"name":"Program Against Cancer Therapeutic Resistance (ProCURE) Institut Catal\u00e0 d'Oncologia, L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Llad\u00f3","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"},{"name":"Department of Surgery Hospital Universitari de Bellvitge L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Mezheyeuski","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology Uppsala University  Uppsala Sweden"},{"name":"Molecular Oncology Group, Vall d'Hebron Institute of Oncology  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1392-8070","authenticated-orcid":false,"given":"David G.","family":"Mollev\u00ed","sequence":"additional","affiliation":[{"name":"Tumoural and Stromal Chemoresistance Group, Oncobell Program, IDIBELL L'Hospitalet de Llobregat  Barcelona Catalonia Spain"},{"name":"Program Against Cancer Therapeutic Resistance (ProCURE) Institut Catal\u00e0 d'Oncologia, L'Hospitalet de Llobregat  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-012-2022-3"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2012-0121"},{"key":"e_1_2_10_4_1","article-title":"Treatment of metastatic colorectal cancer: ASCO guideline","author":"Morris VK","year":"2022","journal-title":"J Clin Oncol"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw235"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt236"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/EDBK_200821"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-022-01859-7"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.334"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1131-x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2022.01.012"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.07.002"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-024-10275-5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2022.02.015"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2022.08.006"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgast-2018-000217"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40688-x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389-020-00283-x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2013.107"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232227"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2528"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02243"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-019-0828-1"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13215545"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.07.015"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108571"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06318-7"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2023.104452"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr-21-519"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000330095"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-84787-5"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI146987"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2021.02.007"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00048.2019"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.655152"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20162024"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.01.004"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.72853"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-19-1384"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jjco.a023174"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.966"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4197"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24166"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22269"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041544"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aba0759"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers8030036"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-2257"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15221"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0078-7"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.104876"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2020.101435"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/25785826.2019.1698261"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0127-6"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02215"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0528-0"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2023.188891"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.708770"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00571-6"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.05.017"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-12-316"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.826391"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2016.1260213"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004320000199"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djac178"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.06.011"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2021.03.012"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01363"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.21037\/qims-21-143"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-021-10119-6"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-019-09661-5"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/jncics\/pkab026"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1593-5_9"}],"container-title":["MedComm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mco2.70000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:52:39Z","timestamp":1741715559000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mco2.70000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,19]]},"references-count":72,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/mco2.70000"],"URL":"https:\/\/doi.org\/10.1002\/mco2.70000","archive":["Portico"],"relation":{},"ISSN":["2688-2663","2688-2663"],"issn-type":[{"value":"2688-2663","type":"print"},{"value":"2688-2663","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,19]]},"article-number":"e70000"}}