{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:50:44Z","timestamp":1790272244102,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173383"],"award-info":[{"award-number":["82173383"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Timely and accurate detection of Philadelphia chromosome\u2013like acute lymphoblastic leukemia (Ph\u2010like ALL)\u2010related fusion gene is essential for treatment decisions. However, due to the complexity of possible gene fusion combinations of Ph\u2010like ALL, current diagnostic workflows face critical limitations: prolonged turnaround (7\u201314 days), high costs, and deficiency in degraded specimens. In this study, we introduce Partial Anchored Capture and Long\u2010Read Sequencing (PACLseq), a nanopore\u2010sequencing\u2010technology\u2010based approach. We designed a detection panel associated with Ph\u2010like ALL, specifically\n                    <jats:italic>ABL2<\/jats:italic>\n                    ,\n                    <jats:italic>CSF1R<\/jats:italic>\n                    ,\n                    <jats:italic>PDGFRB<\/jats:italic>\n                    ,\n                    <jats:italic>JAK2<\/jats:italic>\n                    ,\n                    <jats:italic>ABL1<\/jats:italic>\n                    ,\n                    <jats:italic>EPOR<\/jats:italic>\n                    , and\n                    <jats:italic>CRLF2<\/jats:italic>\n                    as target genes. Validated on 47 clinical samples, PACLseq achieved 93.3% sensitivity and 100% specificity in 26 degraded RNA samples (RIN &gt; 3). Crucially, PACLseq maintained detection accuracy in nine low\u2010RIN samples (RIN \u2264 3) with fragmented transcripts. The method requires only 10\u00a0ng of RNA input, delivers results in 3 days (vs. 7\u201314 days for conventional methods), and reduces costs by 50%. By offering rapid and accurate fusion detection, PACLseq has the potential to significantly improve diagnostic efficiency, facilitate timely treatment decisions, and enhance patient outcomes in the management of Ph\u2010like ALL.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70360","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T06:53:04Z","timestamp":1758091984000},"source":"Crossref","is-referenced-by-count":1,"title":["PACLseq: A Standalone Diagnostic Method for Ph\u2010Like Acute Lymphoblastic Leukemia Using Nanopore Sequencing"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3065-4303","authenticated-orcid":false,"given":"Hang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Hematology, Institute of Hematology West China Hospital of Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Yu","sequence":"additional","affiliation":[{"name":"Qitan Technology Ltd.  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmei","family":"Chen","sequence":"additional","affiliation":[{"name":"Qitan Technology Ltd.  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Jiang","sequence":"additional","affiliation":[{"name":"Qitan Technology Ltd.  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Huo","sequence":"additional","affiliation":[{"name":"Qitan Technology Ltd.  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialin","family":"Li","sequence":"additional","affiliation":[{"name":"Qitan Technology Ltd.  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology, Institute of Hematology West China Hospital of Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Omics Technology and Bioinformatics, Frontiers Science Center for Disease\u2010related Molecular Network, State Key Laboratory of Biotherapy West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808253"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70339-5"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015850"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01620-2"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-06-743252"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1403088"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.192088"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2021.278697"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22995"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17340"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-011-0910-z"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.095372"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.47.6770"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-09-670166"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2018.09.003"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V122.21.826.826"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2017.00258"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09374-9"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16174-z"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-020-07207-4"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02588-5"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08339-5"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-22-1628"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.12.013"},{"issue":"1","key":"e_1_2_12_26_1","first-page":"D817","article-title":"ChimerDB 4.0: An Updated and Expanded Database of Fusion Genes","volume":"48","author":"Jang Y. 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