{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:47:18Z","timestamp":1792010838478,"version":"4.4.0"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA1105602"],"award-info":[{"award-number":["2022YFA1105602"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diabetic wound (DW) represent a common complication of diabetes. Despite advances in regenerative repair utilizing endothelial progenitor cells (EPCs), challenges such as low survival and impaired angiogenic function of EPCs remain. Herein, we explored an effective method to induce injury\u2010induced protection for EPCs and improves their function. This was achieved through cell preconditioning under conditions of nutrient deprivation and high glucose (NDHG), combined with sb431542, a transforming growth factor beta (TGF\u2010\u03b2) signaling inhibitor. Specifically, after three generations of cell passage during preconditioning, umbilical cord\u2010derived endothelial cells (ECs) exhibited characteristics resembling those of EPCs, with over 80% of the cells expressed CD34, a typical marker of EPCs. Notably, these preconditioned EPC\u2010like cells (pEPCs) showed tolerance to pathological environment, as evidenced by robust cell viability, improved antioxidant capacity, and stable tube\u2010forming ability under NDHG condition. The protective effect of preconditioning in pEPCs is partly achieved by activating the PI3K\/AKT pathway to upregulate the expression of Nrf2 and HIF\u20101\u03b1. Importantly, pEPCs exhibited therapeutic potential in two diabetic mouse models\u2010limb ischemia and skin wounds by enhancing blood vessel formation and facilitating tissue repair. Overall, this preconditioning method induced the generation of functionally enhanced pEPCs, providing an alternative source of cells for treating DWs.<\/jats:p>","DOI":"10.1002\/mco2.70364","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T06:40:24Z","timestamp":1756795224000},"source":"Crossref","is-referenced-by-count":5,"title":["Preconditioning With TGF\u2010\u03b2 Inhibitors Enhances Therapeutic Efficacy of Endothelial Progenitor Cells for Wound Healing in Diabetic Mice"],"prefix":"10.1002","volume":"6","author":[{"given":"Dongsheng","family":"Su","sequence":"first","affiliation":[{"name":"Department of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University  Chengdu Sichuan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuyi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Biotherapy Cancer Center and State Key Laboratory 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