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Therefore, in this study, we analyzed heterogeneous immune leukocytes associated with ASH at the single\u2010cell level using high\u2010dimensional single\u2010cell RNA sequencing in alcoholic liver disease (ALD)\u2010induced and healthy control mice. A t\u2010distributed stochastic neighbor embedding plot for dimensionality reduction and 2D visualization was used to visualize heterogeneous immune cell types. Moreover, singleR was used for automated cell annotation to identify the cell types and differentially expressed genes from each cell type and their subsets. We observed a decline in the population of B cells and their subsets, with up and downregulated genes signifying an innate proinflammatory response as an important indication of alcohol\u2010induced liver fibrosis. Additionally, neutrophil deficiency in the alcohol\u2010induced mouse group was associated with ASH. An increase in eosinophils diverts further complications in liver fibrosis, suggesting the functional heterogeneity of granulocyte subsets. Overall, our findings may assist in discovering potential ALD biomarker cell types that are significantly reduced by frequent alcohol exposure and enhance our understanding of the circulating immune leukocytes that lead to alcohol\u2010induced liver fibrosis.<\/jats:p>","DOI":"10.1002\/mco2.70448","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:34:50Z","timestamp":1762148090000},"source":"Crossref","is-referenced-by-count":0,"title":["Discovering Heterogeneous Leukocytes Subsets Associated With Alcoholic Steatohepatitis by scRNAseq Analysis"],"prefix":"10.1002","volume":"6","author":[{"given":"Haribalan","family":"Perumalsamy","sequence":"first","affiliation":[{"name":"Research Institute for Convergence of Basic Science Hanyang University  Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Center For Creative Convergence Education Hanyang University  Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Department of Chemistry College of Natural Sciences Hanyang University Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sehee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Hanyang University Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine Graduate School of Biomedical Science and Engineering Hanyang University  Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Chemistry College of Natural Sciences Hanyang University Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Hanyang Institute of Bioscience and Biotechnology Hanyang University Seoul Republic of Korea"},{"name":"Department of Internal Medicine College of Medicine Hanyang University Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae Won","family":"Jun","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine Graduate School of Biomedical Science and Engineering Hanyang University  Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Hanyang Institute of Bioscience and Biotechnology Hanyang University Seoul Republic of Korea"},{"name":"Department of Internal Medicine College of Medicine Hanyang University Seongdong\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2743-6360","authenticated-orcid":false,"given":"Tae\u2010Hyun","family":"Yoon","sequence":"additional","affiliation":[{"name":"Research Institute for Convergence of Basic Science Hanyang University  Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Department of Chemistry College of Natural Sciences Hanyang University Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Institute For Next\u2010Generation Material Design Hanyang University Seongdong\u2010gu Seoul Republic of Korea"},{"name":"Yoon Idea Lab. 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