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In this study, we applied Visium spatial transcriptomics to generate gene expression maps of rat left ventricles after MIR and identified distinct regional features. The border zones were enriched with phagosome\u2010related genes, incomplete infarct areas showed activation of MAPK, IL\u201017, and osteoclast differentiation pathways, while the infarct cores were characterized by ferroptosis and mitophagy\u2010related genes. To further resolve the cellular basis, we integrated single\u2010cell RNA sequencing with RCTD deconvolution and found immune cell infiltration in infarct zones, neutrophil enrichment in incomplete infarct areas, and smooth muscle cell predominance in border zones. Both spatial and single\u2010cell analyses revealed altered expression of Piezo1, RyR2, MMP2, and SERCA2, which was further validated by Western blot and immunofluorescence co\u2010staining with ACTN2. Pseudotime analysis demonstrated selective enrichment and dynamic activation of Piezo1 in specific cardiomyocyte subclusters. Functional validation using a hypoxia\/reoxygenation model confirmed that reoxygenation induced marked intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    accumulation, which was attenuated by the Piezo1 inhibitor GsMTx4. Together, these findings delineate the spatial heterogeneity of MIR injury, identify Piezo1 as a key mediator of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    dysregulation, and suggest Piezo1 as a potential therapeutic target for myocardial protection.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70537","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T11:15:19Z","timestamp":1767352519000},"source":"Crossref","is-referenced-by-count":3,"title":["Spatial Transcriptional Heterogeneity in the Infarct Core and Its Surrounding Regions Targeting Piezo1 Signals in Rats With Myocardial Ischemia\u2010Reperfusion Injury"],"prefix":"10.1002","volume":"7","author":[{"given":"Zhen","family":"Li","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Hainan General Hospital Hainan Affiliated Hospital of Hainan Medical University  Haikou Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqiong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"He","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duozhi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Hainan General Hospital Hainan Affiliated Hospital of Hainan Medical University  Haikou Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbing","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine Hubei Key Laboratory of Geriatric Anesthesia and Perioperative Brain Health Wuhan Clinical Research Center for Geriatric Anesthesia Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei China"},{"name":"Key Laboratory of Anesthesiology and Resuscitation (Huazhong University of Science and Technology) Ministry of Education  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"issue":"8","key":"e_1_2_12_2_1","first-page":"e153","article-title":"Heart Disease and Stroke Statistics\u20102022 Update: A Report from the American Heart Association","volume":"145","author":"Tsao C. 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