{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:13:36Z","timestamp":1791951216872,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tuberculosis (TB) remains a major global health challenge. In this study, we applied UPLC\u2010MS\/MS lipidomics and data\u2010independent acquisition proteomics to profile plasma from healthy controls, active TB patients, and cured individuals to identify differentially expressed lipids and proteins. Mendelian randomization prioritized phosphatidylcholine (PC) lipids (PC(18:2\/18:2), PC(14:0\/20:4) and PC(18:0\/20:4)) and proteins (haptoglobin [HP], retinol binding protein 4 [RBP4], coagulation factor XIII B subunit [F13B] and inter\u2010alpha\u2010trypsin inhibitor heavy chain 1 [ITIH1]) as candidate diagnostic and cure biomarkers. Binary multi\u2010omics random\u2010forest classifiers constructed with these markers achieved strong diagnostic (AUC = 0.967, 95% CI: 0.928\u20131.000) and cure\u2010monitoring (AUC = 0.981, 95% CI: 0.956\u20131.000) performance, which was further assessed with ten\u2010fold cross\u2010validation. Integration with transcriptomic data and lipid\u2010related gene analysis provided additional molecular support for HP. Independent validation in the GSE34608 cohort (AUC = 0.965) and ELISA verification (AUC = 0.969) confirmed HP's diagnostic utility at gene and protein levels. GSVA enrichment implicated HP in iron homeostasis and immune response pathways, suggesting a role in\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    infection and immune evasion through modulation of host iron metabolism. Overall, we present a robust lipid\u2013protein biomarker panel and accurate multi\u2010omics models for TB diagnosis and monitoring of cure, and propose HP as a promising biomarker and potential therapeutic target. These tools may improve clinical management and treatment evaluation.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70559","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T06:45:35Z","timestamp":1767077135000},"source":"Crossref","is-referenced-by-count":5,"title":["Robust Diagnostic and Therapeutic Biomarkers for Tuberculosis Identified Through Multi\u2010Omics and Mendelian Randomization Analysis"],"prefix":"10.1002","volume":"7","author":[{"given":"Chenglin","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Henan University  Kaifeng Henan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxi","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University  Linhai Zhejiang Province People's Republic of 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