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Herein, this review details the in\u2010depth methodology, updated to date, for the efficient cultivation of organoids. Emphasizing liver organoids, both hepatocyte and cholangiocyte derived, and other abdominal organ systems, such as gut, kidney, and pancreas, we explore the technological challenges researchers are facing nowadays, including how to optimize nutrient delivery, maintain cellular diversity, achieve scalability in the organoid culture system, and high\u2010throughput applications. Addressing those biological and technological complexities, this review aimed at equipping new researchers with practical insights and standardized protocols that will help improve reproducibility and success rates in organoid culture and expand their applications. Furthermore, we discuss current limitations and barriers to clinical translation, highlight key knowledge gaps, and outline emerging innovations, including bioengineering, microfluidic systems, and genetic manipulation, expected to further enhance disease modeling, personalized medicine, and regenerative therapies. Finally, we provide perspective on next\u2010generation technologies that expedite organoid\u2010based discovery and development.<\/jats:p>","DOI":"10.1002\/mco2.70571","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T07:42:14Z","timestamp":1767685334000},"source":"Crossref","is-referenced-by-count":6,"title":["Mastering Organoid Growth: A Complete Guide to Overcoming Methodological Challenges"],"prefix":"10.1002","volume":"7","author":[{"given":"Chunbao","family":"Jiao","sequence":"first","affiliation":[{"name":"Department of Inflammation and Immunity Cleveland Clinic Research Cleveland Clinic Cleveland Ohio USA"},{"name":"Department of Hepatobiliary and Pancreatic Surgery General Surgery Center First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer Faruk","family":"Karakaya","sequence":"additional","affiliation":[{"name":"Department of Inflammation and Immunity Cleveland 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