{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:21:06Z","timestamp":1788402066711,"version":"build-2803163510"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3405200"],"award-info":[{"award-number":["2023YFC3405200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471854"],"award-info":[{"award-number":["82471854"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tumor\u2010derived extracellular vesicles (EVs) are a class of natural nanocarriers with phospholipid bilayers that show great promise as personalized cancer vaccine platforms due to their ability to carry tumor\u2010specific antigens. However, their immunotherapeutic potential is hindered by limited tissue\u2010specific targeting. In this study, we engineered tumor cell\u2010derived EVs using an immunomodulatory peptide, DP7\u2010C, to generate DP7\u2010C engineered EVs (DP\u2010EVs). These DP\u2010EVs exhibited significantly enhanced accumulation in both lymph nodes and tumor tissues. Additionally, they demonstrated improved cellular uptake and facilitated more efficient endosomal escape. To further enhance the therapeutic efficacy, programmed cell death 1 ligand 1 targeting small interfering RNA\u00a0(siPD\u2010L1) was loaded into the DP\u2010EVs, resulting in DP\u2010EVs\/siPD\u2010L1. This formulation enabled concurrent suppression of PD\u2010L1 expression in both dendritic cells (DCs) and tumor cells. In vivo experiments showed that DP\u2010EVs\/siPD\u2010L1 significantly inhibited tumor growth and prolonged survival in tumor\u2010bearing mice. The observed antitumor effect was attributed to the immune activation in the lymph nodes and the remodeling of the immunosuppressive tumor microenvironment (TME). Collectively, our findings demonstrate that DP\u2010EVs\/siPD\u2010L1 functions as an effective therapeutic vaccine, which synergistically activates antitumor immunity and reverses immunosuppression through targeted PD\u2010L1 blockade. This engineered EV platform represents a promising and translatable strategy for cancer immunotherapy.<\/jats:p>","DOI":"10.1002\/mco2.70673","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T05:57:41Z","timestamp":1773122261000},"source":"Crossref","is-referenced-by-count":1,"title":["An Engineered Extracellular Vesicle With Enhanced Tumor and Lymph Nodes Targeting as siRNA Delivery System for Robust Tumor Immunotherapy"],"prefix":"10.1002","volume":"7","author":[{"given":"Yusi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital Sichuan University  Chengdu 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