{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T07:12:22Z","timestamp":1794035542707,"version":"4.8.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270662"],"award-info":[{"award-number":["82270662"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070640"],"award-info":[{"award-number":["82070640"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Liver failure remains a life\u2010threatening syndrome where the available therapeutic options are extremely limited beyond transplantation. This study addresses critical cell source and mechanistic challenges by developing a novel bioartificial liver (BAL) system. We utilized CRISPR\/Cas9 technology to knockout the\n                    <jats:italic>GGTA1<\/jats:italic>\n                    gene in primary porcine hepatocytes to reduce immunogenicity. These hepatocytes were co\u2010cultured with R\u2010spondin1\u2010overexpressing human umbilical vein endothelial cells (R\u2010HUVECs) to form functionally stable liver organoids. In ex vivo study using plasma from patients with acute\u2010on\u2010chronic liver failure (ACLF), the BAL system demonstrated superior detoxification, significantly reducing ammonia and bilirubin levels compared to traditional non\u2010bioartificial liver (NAL) support. Multi\u2010omics analyses revealed that BAL treatment actively restored metabolic homeostasis by promoting branched\u2010chain amino acid (BCAA) metabolism and upregulating lysophosphatidylcholine (LPC) species associated with membrane repair and anti\u2010inflammatory signaling. Significantly, this research demonstrates that unlike the passive physical filtration of NAL, BAL serves as an active biological regulator of systemic metabolism. These findings provide a robust theoretical and practical foundation for the clinical translation of BAL technology, offering a promising strategy to improve outcomes for liver failure patients by modulating systemic metabolism.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70795","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T06:40:59Z","timestamp":1780296059000},"source":"Crossref","is-referenced-by-count":2,"title":["Bioartificial Livers Developed From Gene\u2010Edited Pig Hepatocyte Organoids Improve Amino Acid and Lipid Profiles in the Plasma of Patients With Liver Failure"],"prefix":"10.1002","volume":"7","author":[{"given":"Yuting","family":"He","sequence":"first","affiliation":[{"name":"Department of Pathology Institute of Clinical Pathology Key Laboratory of Transplant Engineering and Immunology NHC West China Hospital Sichuan University  Chengdu China"},{"name":"Department of Pathology West China Second University Hospital Sichuan 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(Sichuan University) Ministry of Education  Chengdu China"},{"name":"Department of Medical Genetics\/Prenatal Diagnostic Center West China Second University Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Clinical Pathology Key Laboratory of Transplant Engineering and Immunology NHC West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanliu","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Clinical Pathology Key Laboratory of Transplant Engineering and Immunology NHC West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Clinical 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