{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T07:07:30Z","timestamp":1784012850866,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Combined immune checkpoint inhibitor (ICI) and chemotherapy is the standard first\u2010line treatment for advanced non\u2010small cell lung cancer (NSCLC) without targetable driver mutations. However, reliable biomarkers predictive of clinical benefit are lacking. We analyzed 54 patients with advanced NSCLC treated first\u2010line with ICI plus chemotherapy. Pretreatment tumor biopsies underwent targeted DNA and RNA\u2010seq. Plasma samples for circulating tumor DNA (ctDNA) profiling were collected at multiple timepoints. Associations between genomic, transcriptomic, and ctDNA features and objective response rate (ORR) and progression\u2010free survival (PFS) were assessed. Genomic analysis revealed that\n                    <jats:italic>LRP1B<\/jats:italic>\n                    mutations predicted longer PFS (HR = 0.35,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.034), whereas\n                    <jats:italic>FBXW7<\/jats:italic>\n                    mutations were associated with shorter PFS (HR = 5.39,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.001). Exploratory transcriptomic analysis identified high\n                    <jats:italic>SPP1<\/jats:italic>\n                    and\n                    <jats:italic>LHCGR<\/jats:italic>\n                    expression as predictors of poor prognosis, while high\n                    <jats:italic>IL24<\/jats:italic>\n                    expression correlated with longer PFS. CD8\n                    <jats:sup>+<\/jats:sup>\n                    effector memory T\u2010cell infiltration was significantly higher in responders (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.029). Longitudinal ctDNA analysis showed that positivity at C2D1 (HR = 5.18,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.004), C3D1 (HR = 17.81,\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001), and C4D1(HR = 3.91,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.018) was associated with inferior PFS. This multi\u2010omics analysis highlights the potential of integrating genomic, transcriptomic, and ctDNA biomarkers to optimize immunotherapy strategies in NSCLC.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70846","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T07:00:09Z","timestamp":1783407609000},"source":"Crossref","is-referenced-by-count":0,"title":["Integrated Genomic, Transcriptomic, and Circulating Biomarkers Predict Benefit to Immune Checkpoint Inhibitor Plus Chemotherapy in Advanced Non\u2010Small Cell Lung Cancer"],"prefix":"10.1002","volume":"7","author":[{"given":"Lailing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Oncology The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei 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Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Oncology The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Respiratory Oncology The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei 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of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Oncology The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Department of Respiratory Oncology Anhui Provincial Cancer Hospital  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"He","sequence":"additional","affiliation":[{"name":"Bozhou Traditional Chinese Medicine Hospital Anhui Province  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shicheng","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Pulmonology Taihe County Traditional Chinese 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