{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:25:20Z","timestamp":1787124320854,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["MD763947"],"award-info":[{"award-number":["MD763947"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) remains a leading cause of cancer\u2010related mortality worldwide, largely due to limitations in early diagnosis and effective treatment. To address these challenges, we developed a multifunctional probiotic nanoplatform, EcNA@Cur\u2010Lip\u2010FA, that integrates real\u2010time diagnosis, targeted therapy, and immune modulation for enhanced CRC treatment. Specifically, this system leverages genetically engineered\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Nissle 1917 (EcN) encoding acoustic reporter genes (ARGs) to produce ultrasound (US)\u2010responsive gas vesicles (GVs), enabling real\u2010time, noninvasive tumor imaging. Simultaneously, folic acid (FA)\u2010modified curcumin\u2010loaded liposomes (Cur\u2010Lip\u2010FA) are co\u2010delivered with EcN, enhancing tumor\u2010specific accumulation and therapeutic precision. After being taken up by tumor cells, curcumin is released locally upon exposure to US irradiation, generating reactive oxygen species (ROS), inducing immunogenic cell death (ICD), and activating the cGAS\u2010STING pathway to promote dendritic cells (DCs) maturation and robust CD4\n                    <jats:sup>+<\/jats:sup>\n                    \/CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses. Additionally, EcNA@Cur\u2010Lip\u2010FA downregulates angiogenesis\u2010related factors (VEGF, VEGFR1, and ANGPT1), thereby inhibiting tumor neovascularization. In vitro and in vivo studies demonstrated that EcNA@Cur\u2010Lip\u2010FA enables precise tumor localization, dynamic imaging, and effective suppression of both primary CRC and metastatic liver lesions with favorable biocompatibility. This multifunctional platform offers a promising strategy for integrating diagnosis and treatment, paving the way for next\u2010generation CRC theranostics.\n                  <\/jats:p>","DOI":"10.1002\/mco2.70871","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:58:23Z","timestamp":1784271503000},"source":"Crossref","is-referenced-by-count":0,"title":["Genetically Engineered Probiotic Nanoplatform for Ultrasound\u2010Enhanced Colorectal Cancer Therapy via Targeted Delivery, Immune Modulation, and Anti\u2010Angiogenesis"],"prefix":"10.1002","volume":"7","author":[{"given":"Jie","family":"Long","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongrui","family":"Shang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong\u2010Hong Kong Joint Laboratory for RNA Medicine Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuezhong","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2579-6220","authenticated-orcid":false,"given":"Yunfang","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong\u2010Hong Kong Joint Laboratory for RNA Medicine Sun Yat\u2010sen Memorial Hospital, Sun Yat\u2010sen University  Guangzhou China"},{"name":"Artificial Intelligence Cross Disciplinary Research Institute, Faculty of Medicine, Faculty of Innovation Engineering, School of Computer Science and Engineering Macau University of Science and Technology  Macau China"},{"name":"Guangdong Provincial Key Laboratory of Cancer Pathogenesis and Precision Diagnosis and Treatment, AI Big Data Laboratory, Shenshan Medical Center Memorial Hospital of Sun Yat\u2010Sen University  Shanwei China"},{"name":"The First Affiliated Hospital, Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cancer Pathogenesis and Precision Diagnosis and Treatment, AI Big Data Laboratory, Shenshan Medical Center Memorial Hospital of Sun Yat\u2010Sen University  Shanwei China"},{"name":"School of Medicine University of Dundee  Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Baptista\u2010Hon","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cancer Pathogenesis and Precision Diagnosis and Treatment, AI Big Data Laboratory, Shenshan Medical Center Memorial Hospital of Sun Yat\u2010Sen University  Shanwei China"},{"name":"School of Medicine University of Dundee  Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic Chi\u2010Chung","family":"FOO","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Hong Kong Hong Kong, SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lui","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Hong Kong Hong Kong, SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renchuan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Tong","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhong","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, Colorectal and Anal Disease Unit, Guangxi Key Laboratory of Basic and Translational Research for Colorectal Cancer Guangxi Medical University Cancer Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Artificial Intelligence Cross Disciplinary Research 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