{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:25:38Z","timestamp":1787124338079,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270944"],"award-info":[{"award-number":["32270944"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alveolar macrophages (AMs) serves as a frontline innate barrier against pulmonary bacterial invasion. The AM pool comprises tissue\u2010resident AMs (TR\u2010AMs) and monocyte\u2010derived AMs (Mo\u2010AMs); yet how vaccination remodels the mixed AM pool for long\u2010term antimicrobial defense against multidrug\u2010resistant bacteria remains poorly understood. In this work, we applied intranasal inactivated whole\u2010cell (IWC) vaccination against\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    and systematically dissected AM population dynamics at cellular and molecular levels. Vaccination reshaped the AM pool by recruiting CD11B\n                    <jats:sup>+<\/jats:sup>\n                    CD13\n                    <jats:sup>+<\/jats:sup>\n                    Mo\u2010AMs that gradually acquire a TR\u2010AMs\u2010like phenotype and training TR\u2010AMs via sustained transcriptional adaptations. Functionally, the IWC\u2010remodeled AM pool exhibited enhanced antigen presentation, TNF\u2010\u03b1 secretion, and phagocytosis, accompanied by metabolic rewiring, thereby conferring durable protection against\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    infection. ATAC\u2010seq revealed immune\u2010related chromatin remodeling and enrichment of transcription factors, including ETS, IRF, and bZIP family members. Similar AM pool remodeling was observed following immunization with IWC vaccines against\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    , suggesting parallel innate responses across multiple common respiratory bacterial vaccines. Collectively, our study delineates the single\u2010cell transcriptional and epigenetic landscapes of vaccine\u2010remodeled AM subsets, providing mechanistic insights to guide the development of AM\u2010targeted prophylactic vaccines against multidrug\u2010resistant\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/mco2.70887","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T08:42:34Z","timestamp":1785746554000},"source":"Crossref","is-referenced-by-count":0,"title":["Vaccination Drives Alveolar Macrophages Pool Remodeling via Training Resident Cells and Recruiting Monocyte\u2010Derived Cells to Defend Against Multidrug\u2010Resistant\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1002","volume":"7","author":[{"given":"Xiaomin","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Biopharmaceuticals West China Hospital Sichuan 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