{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:44:51Z","timestamp":1792043091205,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302800"],"award-info":[{"award-number":["82302800"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2026GXNSFAA00640133"],"award-info":[{"award-number":["2026GXNSFAA00640133"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MedComm"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sepsis has high intensive care unit mortality because biomarkers for early reversible pathological events are lacking. Targeted nuclear degranulation, the rate\u2010limiting step in neutrophil extracellular trap formation, refers to azurophilic granule fusion with the nuclear membrane. As an exclusive granule membrane marker, CD63 enrichment in nuclear membranes directly reflects this early pathogenic process. We developed a flow cytometry assay to quantify nuclear membrane CD63 (nmCD63) and validated its clinical utility in a two\u2010center prospective cohort with strict independent external validation. Assay protocols, diagnostic cutoffs, and combined models were established for the derivation cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 180) and applied without recalibration to the validation cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 76) comprising 256 participants. nmCD63 levels increased progressively with disease severity. They differentiated non\u2010infectious systemic inflammation from non\u2010severe infections (area under the curve [AUC] = 0.794) and outperformed C\u2010reactive protein and procalcitonin levels. Combined with the Sequential Organ Failure Assessment score, nmCD63 achieved an AUC of 0.943 for identifying sepsis in patients with a confirmed infection. Dynamic nmCD63 shifts preceded changes in all conventional biomarkers, and 7\u2010day trajectories enabled reliable prognostic stratification. This study translates targeted nuclear degranulation as a practical biomarker with dual diagnostic and prognostic value for sepsis in critically ill patients.\n                  <\/jats:p>","DOI":"10.1002\/mco2.71007","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:54:03Z","timestamp":1789628043000},"source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Changes of Neutrophil Nuclear Membrane CD63 as a Potential Biomarker for Early Adjunctive Diagnosis and Prognostic Evaluation of Sepsis in Critically Ill Patients: A Prospective Cohort Study"],"prefix":"10.1002","volume":"7","author":[{"given":"Xingxin","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linbin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haihua","family":"Lyu","sequence":"additional","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Jining Medical University  Jining Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xucheng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Burns Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-024-07694-z"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-024-01244-9"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092385"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-025-05283-0"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17231"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202301780R"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcmedi\/pbae028"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2025.106100"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEJ.0000000000001235"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)00658-4"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-116285"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.36"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.23916"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12196171"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2024.12.004"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S337691"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.39367"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.1545"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3350\/cmh.2023.0563"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01933-x"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.2147\/ITT.S569962"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2109-7"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aar6676"}],"container-title":["MedComm"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mco2.71007","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mco2.71007","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mco2.71007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:49:31Z","timestamp":1792039771000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mco2.71007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/mco2.71007"],"URL":"https:\/\/doi.org\/10.1002\/mco2.71007","archive":["Portico"],"relation":{},"ISSN":["2688-2663","2688-2663"],"issn-type":[{"value":"2688-2663","type":"print"},{"value":"2688-2663","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]},"article-number":"e71007"}}