{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:15:14Z","timestamp":1790036114258,"version":"4.0.1"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T00:00:00Z","timestamp":1535587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JP17dm0107146"],"award-info":[{"award-number":["JP17dm0107146"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["movementdisorders.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Movement Disorders"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Parkinson's disease is caused by dopamine deficiency in the striatum, which is a result of loss of dopamine neurons from the substantia nigra pars compacta. There is a consensus that a subpopulation of nigral dopamine neurons that expresses the calcium\u2010binding protein calbindin is selectively invulnerable to parkinsonian insults. The objective of the present study was to test the hypothesis that dopamine neuron degeneration might be prevented by viral vector\u2010mediated gene delivery of calbindin into the dopamine neurons that do not normally contain it.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A calbindin\u2010expressing adenoviral vector was injected into the striatum of macaque monkeys to be conveyed to cell bodies of nigral dopamine neurons through retrograde axonal transport, or the calbindin\u2010expressing lentiviral vector was injected into the nigra directly because of its predominant uptake from cell bodies and dendrites. The animals in which calbindin was successfully recruited into nigral dopamine neurons were administered systemically with MPTP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the monkeys that had received unilateral vector injections, parkinsonian motor deficits, such as muscular rigidity and akinesia\/bradykinesia, appeared predominantly in the limbs corresponding to the non\u2010calbindin\u2010recruited hemisphere after MPTP administration. Data obtained from tyrosine hydroxylase immunostaining and PET imaging for the dopamine transporter revealed that the nigrostriatal dopamine system was preserved better on the calbindin\u2010recruited side. Conversely, on the non\u2010calbindin\u2010recruited control side, many more dopamine neurons expressed \u03b1\u2010synuclein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present results indicate that calbindin recruitment into nigral dopamine neurons protects against the onset of parkinsonian insults, thus providing a novel approach to PD prevention. \u00a9 2018 International Parkinson and Movement Disorder Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/mds.107","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T16:10:20Z","timestamp":1535645420000},"page":"200-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Recruitment of calbindin into nigral dopamine neurons protects against MPTP\u2010Induced parkinsonism"],"prefix":"10.1002","volume":"34","author":[{"given":"Ken\u2010ichi","family":"Inoue","sequence":"first","affiliation":[{"name":"Systems Neuroscience Section, Department of Neuroscience, Primate Research Institute Kyoto University  Inuyama Aichi Japan"},{"name":"Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan Organization for Medical Research  Fuchu Tokyo Japan"},{"name":"PRESTO, Japan Science and Technology Agency  Kawaguchi Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehiro","family":"Miyachi","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan Organization for Medical Research  Fuchu Tokyo Japan"},{"name":"Cognitive Neuroscience Section, Department of Neuroscience, Primate Research Institute Kyoto University  Inuyama Aichi 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