{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:44:16Z","timestamp":1761007456021,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,3,4]],"date-time":"2004-03-04T00:00:00Z","timestamp":1078358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Movement Disorders"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Approximately 5% of patients with cervical dystonia receiving repeated botulinum neurotoxin A (BoNT\/A) injections develop secondary loss of treatment benefit. Currently available tests to directly detect neutralizing BoNT\/A antibodies (BoNT\/A\u2010AB) are either expensive or time consuming. To establish a simple, clinically useful test for antibody detection, we adapted the ninhydrin sweat test (NST). Eighteen dystonic patients with secondary nonresponse and clinically suspected BoNT\/A\u2010AB formation were tested for BoNT\/A\u2010AB in the mouse diaphragm test (MDT). In addition, the size of the anhidrotic area was determined by the NST 21 days after an intradermal dose of 10 U Dysport into the hypothenar region of the left palm. In nine patients, positive BoNT\u2010AB titers were found in the MDT. There was a significant correlation between the BoNT\/A\u2010AB titers and the anhidrotic area (Spearman's rho = \u22120.9, <jats:italic>P<\/jats:italic> &lt; 0.0001). Both tests provided comparably good results with respect to qualitative antibody detection. In the clinical situation of secondary nonresponse to BoNT\/A therapy, the economical NST may be a helpful tool to detect neutralizing BoNT\/A\u2010AB. \u00a9 2004 Movement Disorder Society<\/jats:p>","DOI":"10.1002\/mds.20073","type":"journal-article","created":{"date-parts":[[2004,3,25]],"date-time":"2004-03-25T12:35:18Z","timestamp":1080218118000},"page":"943-947","source":"Crossref","is-referenced-by-count":14,"title":["Ninhydrin sweat test: A simple method for detecting antibodies neutralizing botulinum toxin type 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