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We tried to find molecular and cellular differences in dopaminergic (DA) neurons derived from healthy subjects and idiopathic PD patients with or without rest tremor at onset.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We measured the expression of genes controlling dopamine synthesis, sequestration, and catabolism as well as the levels of corresponding metabolites and reactive oxygen species in midbrain DA neurons differentiated from induced pluripotent stem cells (iPSCs) of healthy subjects and PD patients with or without rest tremor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant differences in DA\u2010related gene expression, metabolites, and oxidative stress were found between midbrain DA neurons derived from healthy subjects and patients with PD. DA neurons derived from PD patients with or without rest tremor at onset exhibited significant differences in the levels of some of these transcripts, metabolites, and oxidative stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The unique combination of these quantifiable molecular and cellular traits in iPSC\u2010derived midbrain DA neurons can distinguish healthy subjects from idiopathic PD patients and segregate PD patients with or without rest tremor at onset. The strategy may be used to develop an objective diagnostic test for PD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mds.28786","type":"journal-article","created":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T11:51:00Z","timestamp":1632657060000},"page":"70-79","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Molecular Features of Parkinson's Disease in Patient\u2010Derived Midbrain Dopaminergic Neurons"],"prefix":"10.1002","volume":"37","author":[{"given":"Yong","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics State University of New York at Buffalo  Buffalo New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houbo","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics State University of New York at Buffalo  Buffalo New York 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