{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:43:52Z","timestamp":1776930232222,"version":"3.51.2"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008280","name":"Universite Angers","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["movementdisorders.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Movement Disorders"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The architecture and composition of glial (GCI) and neuronal (NCI) \u03b1\u2010synuclein inclusions observed in multiple system atrophy (MSA) remain to be precisely defined to better understand the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we used stochastic optical reconstruction microscopy (STORM) to characterize the nanoscale organization of glial (GCI) and neuronal (NCI) \u03b1\u2010synuclein inclusions in cryopreserved brain sections from MSA patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>STORM revealed a dense cross\u2010linked internal structure of \u03b1\u2010synuclein in all GCI and NCI. The internal architecture of hyperphosphorylated \u03b1\u2010synuclein (p\u2010\u03b1Syn) inclusions was similar in glial and neuronal cells, suggesting a common aggregation mechanism. A similar sequence of p\u2010\u03b1Syn stepwise intracellular aggregation was defined in oligodendrocytes and neurons, starting from the perinuclear area and growing inside the cells. Consistent with this hypothesis, we found a higher mitochondrial density in GCI and NCI compared to oligodendrocytes and neurons from unaffected donors (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), suggesting an active recruitment of the organelles during the aggregation process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These first STORM images of GCI and NCI suggest stepwise \u03b1\u2010synuclein aggregation in MSA. \u00a9 2024 The Authors. <jats:italic>Movement Disorders<\/jats:italic> published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mds.29744","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T05:50:47Z","timestamp":1707976247000},"page":"723-728","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>Stochastic Optical Reconstruction Microscopy<\/scp> Imaging of Multiple System Atrophy Inclusions Suggests Stepwise \u03b1\u2010Synuclein Aggregation"],"prefix":"10.1002","volume":"39","author":[{"given":"Beno\u00eet","family":"Vovard","sequence":"first","affiliation":[{"name":"Univ Angers, Equipe MitoLab, Unit\u00e9 MitoVasc, Inserm U1083, CNRS 6015, SFR ICAT  Angers France"},{"name":"Laboratoire de neurobiologie et neuropathologie Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexia","family":"Bodin","sequence":"additional","affiliation":[{"name":"Univ Angers, Equipe MitoLab, Unit\u00e9 MitoVasc, Inserm U1083, CNRS 6015, SFR ICAT  Angers France"},{"name":"Laboratoire de neurobiologie et neuropathologie Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Gouju","sequence":"additional","affiliation":[{"name":"Laboratoire de neurobiologie et neuropathologie Centre Hospitalier Universitaire d'Angers  Angers France"},{"name":"MINT, UMR Inserm 1066, CNRS 6021  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"de Guilhem de Lataillade","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD  Nantes France"},{"name":"Service de Neurologie, CHU Nantes, Inserm U1235 Nantes  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8792-2582","authenticated-orcid":false,"given":"Pascal","family":"Derkinderen","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD  Nantes France"},{"name":"Service de Neurologie, CHU Nantes, Inserm U1235 Nantes  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Etcharry\u2010Bouyx","sequence":"additional","affiliation":[{"name":"Service de Neurologie, centre m\u00e9moire de ressource et de recherche Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Chauvir\u00e9","sequence":"additional","affiliation":[{"name":"Service de Neurologie, centre m\u00e9moire de ressource et de recherche Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Guillet\u2010Pichon","sequence":"additional","affiliation":[{"name":"Service de Neurologie, centre m\u00e9moire de ressource et de recherche Centre Hospitalier Universitaire d'Angers  Angers France"},{"name":"Service de Neurologie, centre de r\u00e9f\u00e9rence des maladies neurog\u00e9n\u00e9tiques Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Verny","sequence":"additional","affiliation":[{"name":"Univ Angers, Equipe MitoLab, Unit\u00e9 MitoVasc, Inserm U1083, CNRS 6015, SFR ICAT  Angers France"},{"name":"Service de Neurologie, centre de r\u00e9f\u00e9rence des maladies neurog\u00e9n\u00e9tiques Centre Hospitalier Universitaire d'Angers  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck","family":"Letournel","sequence":"additional","affiliation":[{"name":"Laboratoire de neurobiologie et neuropathologie Centre Hospitalier Universitaire d'Angers  Angers France"},{"name":"MINT, UMR Inserm 1066, CNRS 6021  Angers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Lenaers","sequence":"additional","affiliation":[{"name":"Univ Angers, Equipe MitoLab, Unit\u00e9 MitoVasc, Inserm U1083, CNRS 6015, SFR ICAT  Angers France"},{"name":"Service 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