{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:47:02Z","timestamp":1792597622403,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017599","name":"Science and Technology Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2024C03100"],"award-info":[{"award-number":["2024C03100"]}],"id":[{"id":"10.13039\/501100017599","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82450114"],"award-info":[{"award-number":["82450114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["movementdisorders.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Movement Disorders"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Recent studies have reported associations between expanded\n                      <jats:italic>HSF1<\/jats:italic>\n                      intronic variable number tandem repeats (VNTRs) and essential tremor (ET), necessitating independent validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed\n                      <jats:italic>HSF1<\/jats:italic>\n                      VNTR lengths in 720 ET patients and 507 controls using fluorescence amplicon length analysis. Sequence composition and interruption patterns were characterized using long\u2010read sequencing (LRS) data from 26 ET patients and 29 controls harboring expanded VNTRs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Expanded VNTRs showed similar frequencies in ET patients and controls at both 700\u2009base pairs (bp) (5.9% vs. 4.3%,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.22) and 550\u2009bp thresholds (13.7% vs. 10.8%,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.14). LRS refined the motif pattern from (CCCCGCNCCGCCT)n\/(CCNCGCCT)n to (CNCCGCNCCGCCT)n\/(CNNCGCCT)n. Both patients and controls showed identical repeat configurations with predominantly CC interruptions accompanied by CCCCG interruptions (75% in patients, 41.4% in controls). Self\u2010organizing map analysis revealed no clustering differences between groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In our study, expanded\n                      <jats:italic>HSF1<\/jats:italic>\n                      VNTRs are not enriched in ET patients, with no distinguishing sequence features between patients and controls. Further validation in additional large cohorts is warranted. \u00a9 2026 International Parkinson and Movement Disorder Society.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mds.70304","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T09:19:39Z","timestamp":1775207979000},"page":"2777-2782","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Independent Validation and Structural Characterization of Expanded\n                    <scp>\n                      <i>HSF1<\/i>\n                    <\/scp>\n                    Intronic Variable Number Tandem Repeats in Essential Tremor"],"prefix":"10.1002","volume":"41","author":[{"given":"Xiaosheng","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou 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