{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T02:00:48Z","timestamp":1761789648288,"version":"3.41.2"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Clinical Medical Research of Peritoneal Cancer of Wuhan","award":["2015060911020462"],"award-info":[{"award-number":["2015060911020462"]}]},{"name":"Hubei Natural Science Foundation","award":["2019CFB197"],"award-info":[{"award-number":["2019CFB197"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circular RNAs (circRNAs) are a class of newly discovered RNAs that attach great importance to modulate gene expression and biological function. Nonetheless, in gastric cancer (GC), the expression and function of circRNA are much less explored. In this study, circ_0000267 expression in GC was investigated and the function and mechanism of circ_0000267 was probed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Quantitative real\u2010time PCR (qRT\u2010PCR) was employed to detect circ_0000267, miR\u2010503\u20105p, and <jats:italic>HMGA2<\/jats:italic> expression. Immunohistochemistry and western blot were adopted to detect HMGA2 and epithelial\u2013mesenchymal transition (EMT)\u2010related proteins (E\u2010cadherin and N\u2010cadherin) expression in GC tissues and cells, respectively. GC cell lines with circ_0000267 overexpressed and knocked down were constructed, and CCK\u20108 assay, BrdU assay, scratch healing assay, and transwell assay were employed to assess the effect of circ_0000267 on the proliferation and metastasis of GC cells. Besides, dual\u2010luciferase reporter gene assay was adopted to verify the targeting relationship between circ_0000267 and miR\u2010503\u20105p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Circ_0000267 showed a significant upregulation in GC tissues and cell lines, and its high expression level was extremely linked to the increased tumor diameter and local lymph node metastasis. Circ_0000267 overexpression accelerated GC cell proliferation, metastasis, and EMT processes, while knocking down circ_0000267 led to the opposite effect. From the perspective of mechanism, circ_0000267 promoted the progression of GC through adsorbing miR\u2010503\u20105p and upregulating <jats:italic>HMGA2<\/jats:italic> expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Circ_0000267 is an oncogenic circRNA that affects the progression of GC, which participates in promotion of GC proliferation, migration, invasion, and EMT via modulating the miR\u2010503\u20105p\/<jats:italic>HMGA2<\/jats:italic> axis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1093","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T08:08:56Z","timestamp":1576570136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Circ_0000267 promotes gastric cancer progression via sponging MiR\u2010503\u20105p and regulating <i>HMGA2<\/i> expression"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3720-8932","authenticated-orcid":false,"given":"Xiaopeng","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Zhongnan Hospital Wuhan University Wuhan Hubei China"},{"name":"Hubei Key Laboratory of Tumor Biological Behaviors Wuhan Hubei China"},{"name":"Hubei Cancer Clinical Study Center Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayan","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Zhongnan Hospital Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangdong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thyroid and Breast Surgery Zhongnan Hospital Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology Zhongnan Hospital Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2018.12.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.02.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1814-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.04.017"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1006-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2271"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1759-7714.12476"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/ott.S208235"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2019.6962"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0158-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.Cd-19-0442"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-019-0875-1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.5255"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20181900"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12648"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.02.111"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2019-0121"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28397"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.07.024"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27714"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28411"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-0958-6"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8050639"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.246"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00824"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.9773"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02494-6"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3233\/cbm-170585"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2019.07.045"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v11.i6.459"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10080258"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-019-0933-0"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2019.05.016"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-019-1041-z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgw029"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29050"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00332"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2018.1553787"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0719-3"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-018-0935-5"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101254"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41417-019-0118-6"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.03.035"}],"container-title":["Molecular Genetics &amp; Genomic Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmgg3.1093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mgg3.1093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T09:12:43Z","timestamp":1708506763000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mgg3.1093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,17]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/mgg3.1093"],"URL":"https:\/\/doi.org\/10.1002\/mgg3.1093","archive":["Portico"],"relation":{},"ISSN":["2324-9269","2324-9269"],"issn-type":[{"type":"print","value":"2324-9269"},{"type":"electronic","value":"2324-9269"}],"subject":[],"published":{"date-parts":[[2019,12,17]]},"assertion":[{"value":"2019-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1093"}}