{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T22:43:14Z","timestamp":1786574594418,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Guidance Fund from Renmin hospital of Wuhan University","award":["RMYD2018M76"],"award-info":[{"award-number":["RMYD2018M76"]}]},{"name":"Innovation Project funding from wuhan university school of medicine","award":["TFZZ2018015"],"award-info":[{"award-number":["TFZZ2018015"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Anhydramnios results from the poor development of the placenta or problems with intrauterine development of the kidneys or urinary tract. Complete lack of amniotic fluid indicates a severe problem with the organs of the urinary system. The genes associated with anhydramnios show very diversity and are not yet well defined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole\u2010exome sequencing (WES) was used for an aborted male case around the 20th week of gestation diagnosed with anhydramnios. The resulted deleterious variants were verified by Sanger sequencing. Pathogenicity of deleterious variants was explored by in silico analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A maternally inherited deleterious frameshift variant, c.1454_1455insC, p.(S486Ffs29) in exon 9 and two paternally inherited missense variants c.1037C\u00a0&gt;\u00a0G, p.(Ser346Trp) in exon 7\u00a0and c.1465A\u00a0&gt;\u00a0G, p.(Asn489Asp) in exon 9\u00a0of <jats:italic>Angiotensin\u2010I\u2010Converting Enzyme<\/jats:italic> (<jats:italic>ACE<\/jats:italic>) gene were found and confirmed by Sanger sequencing. c.1454_1455insC, p.(S486Ffs29) and c.1037C\u00a0&gt;\u00a0G, p.(Ser346Trp) were identified as two novel compound heterozygous deleterious variants. The pathogenicity of these deleterious variants was determined by in silico analysis and both the deleterious variants disrupt the structure of the ACE protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Two novel compound heterozygous variants were identified in the case with anhydramnios, which may be associated with pathogenicity of anhydramnios. Our data also revealed that the WES approach may provide helpful information for genetic counseling of the families with anhydramnios.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1239","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T06:36:34Z","timestamp":1587710194000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Two novel deleterious variants of <i>Angiotensin\u2010I\u2010converting Enzyme<\/i> gene identified in a family with recurrent anhydramnios"],"prefix":"10.1002","volume":"8","author":[{"given":"Jingwei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Bin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of obstetrics Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bi\u2010Hua","family":"Tan","sequence":"additional","affiliation":[{"name":"Pennsylvania State University College of Medicine and Hershey Medical center Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"McGrath","sequence":"additional","affiliation":[{"name":"Pennsylvania State University College of Medicine and Hershey Medical center Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3922-2113","authenticated-orcid":false,"given":"Gayle M.","family":"Smink","sequence":"additional","affiliation":[{"name":"Pennsylvania State University College of Medicine and Hershey Medical center Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4081-2543","authenticated-orcid":false,"given":"Chunhua","family":"Song","sequence":"additional","affiliation":[{"name":"Pennsylvania State University College of Medicine and Hershey Medical center Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqing","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Renmin Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1970.tb03418.x"},{"issue":"7","key":"e_1_2_11_3_1","first-page":"20","article-title":"Predicting functional effect of human missense mutations using PolyPhen\u20102","volume":"7","author":"Adzhubei I.","year":"2013","journal-title":"Curr Protoc Hum Genet Chapter"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ckj\/sfw057"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970101005"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv195"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr330"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2205-0140.2013.tb00167.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1623"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21661"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2827"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-013-2480-1"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1360163"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2016.08.016"},{"key":"e_1_2_11_15_1","first-page":"77","article-title":"Polyhydramnios and oligohydramnios","volume":"80","author":"Jeffcoate T. 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