{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:58:21Z","timestamp":1790481501647,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007458","name":"Qatar Foundation","doi-asserted-by":"publisher","award":["28\u20106\u20107\u201017"],"award-info":[{"award-number":["28\u20106\u20107\u201017"]}],"id":[{"id":"10.13039\/100007458","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007458","name":"Qatar Foundation","doi-asserted-by":"publisher","award":["4\u2010157\u20103\u2013052"],"award-info":[{"award-number":["4\u2010157\u20103\u2013052"]}],"id":[{"id":"10.13039\/100007458","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several studies showed that aberrant DNA methylation is involved in leukemia and cancer pathogenesis. Protein tyrosine phosphatase receptor gamma (PTPRG) expression is a natural inhibitory mechanism that is downregulated in chronic myeloid leukemia (CML) disease. The mechanism behind its downregulation has not been fully elucidated yet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to investigate the CpG methylation status at the <jats:italic>PTPRG<\/jats:italic> locus in CML patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood samples from CML patients at time of diagnosis [no tyrosine kinase inhibitors (TKIs)] (<jats:italic>n<\/jats:italic>\u00a0=\u00a013), failure to (TKIs) treatment (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) and healthy controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a06) were collected. DNA was extracted and treated with bisulfite treatment, followed by PCR, sequencing of 25 CpG sites in the promoter region and 26 CpG sites in intron\u20101 region of <jats:italic>PTPRG<\/jats:italic>. The bisulfite sequencing technique was employed as a high\u2010resolution method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CML groups (new diagnosed and failed treatment) showed significantly higher methylation levels in the promoter and intron\u20101 regions of <jats:italic>PTPRG<\/jats:italic> compared to the healthy group. There were also significant differences in methylation levels of CpG sites in the promoter and intron\u20101 regions amongst the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Aberrant methylation of <jats:italic>PTPRG<\/jats:italic> is potentially one of the possible mechanisms of <jats:italic>PTPRG<\/jats:italic> downregulation detected in CML.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1319","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T07:03:56Z","timestamp":1595487836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Aberrant DNA methylation of <i>PTPRG<\/i> as one possible mechanism of its under\u2010expression in CML patients in the State of Qatar"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7647-856X","authenticated-orcid":false,"given":"Mohamed A.","family":"Ismail","sequence":"first","affiliation":[{"name":"School of Life Science, Pharmacy and Chemistry Faculty of Science, Engineering &amp; ComputingFaculty of Science, Engineering &amp; Computing Kingston University London Kingston\u2010Upon\u2010Thames UK"},{"name":"Interim Translational Research Institute (iTRI) Hamad Medical Corporation (HMC) Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthanna","family":"Samara","sequence":"additional","affiliation":[{"name":"Department of Psychology Kingston University London Kingston upon Thames, London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Al Sayab","sequence":"additional","affiliation":[{"name":"Interim Translational Research Institute (iTRI) Hamad Medical Corporation (HMC) Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Alsharshani","sequence":"additional","affiliation":[{"name":"Diagnostic Genetics Division (DGD) Department of Laboratory Medicine and Pathology (DLMP) Hamad Medical Corporation (HMC) Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Yassin","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology National Centre for Cancer Care and Research Hamad Medical Corporation (HMC) Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Govindarajulu","family":"Varadharaj","sequence":"additional","affiliation":[{"name":"Genetrics Inc Dubai United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Vezzalini","sequence":"additional","affiliation":[{"name":"General Pathology Division Department of Medicine University of Verona Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Tomasello","sequence":"additional","affiliation":[{"name":"Wexner Medical Center Biomedical Research Tower The Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Monne","sequence":"additional","affiliation":[{"name":"Centro di Diagnostica Biomolecolare e Citogenetica Emato\u2010Oncologica San Francesco\u201d Hospital Nuoro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisham","family":"Morsi","sequence":"additional","affiliation":[{"name":"Quality of Life unit National Center for Cancer Care and Research, (NCCCR) Hamad Medical Corporation (HMC) Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6757-1922","authenticated-orcid":false,"given":"M. Walid","family":"Qoronfleh","sequence":"additional","affiliation":[{"name":"World Innovation Summit for Healthcare (WISH) Qatar Foundation Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatem","family":"Zayed","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Biomedical Research Center College of Health Sciences QU Health Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Cook","sequence":"additional","affiliation":[{"name":"School of Life Science, Pharmacy and Chemistry Faculty of Science, Engineering &amp; ComputingFaculty of Science, Engineering &amp; Computing Kingston University London Kingston\u2010Upon\u2010Thames UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Sorio","sequence":"additional","affiliation":[{"name":"General Pathology Division Department of Medicine University of Verona Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5509-7415","authenticated-orcid":false,"given":"Helmout","family":"Modjtahedi","sequence":"additional","affiliation":[{"name":"School of Life Science, Pharmacy and Chemistry Faculty of Science, Engineering &amp; ComputingFaculty of Science, Engineering &amp; Computing Kingston University London Kingston\u2010Upon\u2010Thames UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5739-1135","authenticated-orcid":false,"given":"Nader I.","family":"Al\u2010Dewik","sequence":"additional","affiliation":[{"name":"School of Life Science, Pharmacy and Chemistry Faculty of Science, Engineering &amp; ComputingFaculty of Science, Engineering &amp; Computing Kingston University London Kingston\u2010Upon\u2010Thames UK"},{"name":"Qatar Medical Genetic Center (QMGC) Hamad General Hospital (HGH) and Interim Translational Research Institute (iTRI) Hamad Medical Corporation (HMC) Doha Qatar"},{"name":"College of Health and Life Science (CHLS) Hamad Bin Khalifa University (HBKU) Doha Qatar"},{"name":"Department of Pediatrics Women's Wellness and Research Center (WWRC) HMC Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"1","article-title":"Current perspectives for the treatment of chronic myeloid leukemia","volume":"49","author":"Aladag E.","year":"2019","journal-title":"Turkish Journal of Medical Sciences"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.5339\/qfarf.2011.BMP52"},{"key":"e_1_2_12_4_1","first-page":"BMP51","article-title":"Elevation of alpha acid glycoprotein (AGP) does not correlate with the resistance of chronic myeloid leukaemia (CML) to imatinib mesylate (IM)","volume":"2011","author":"Al\u2010Dewik N. I. E.","year":"2011","journal-title":"Hamad bin Khalifa University Press (HBKU Press)"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.5339\/connect.2014.13"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.5339\/connect.2014.24"},{"key":"e_1_2_12_7_1","first-page":"63","article-title":"Studying the impact of presence of alpha acid glycoprotein and protein glycoprotein in chronic myeloid leukemia patients treated with imatinib mesylate in the state of Qatar","volume":"7","author":"Al\u2010Dewik N. I.","year":"2015","journal-title":"Biomark Cancer"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V128.22.5427.5427"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.4137\/cmo.s32822"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(07)70342-X"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-501569"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0562-1"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-018-9841-1"},{"key":"e_1_2_12_14_1","volume-title":"Primer Design and Search Tool","author":"BISEARCH","year":"2019"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V110.11.2122.2122"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0904"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.10.3343"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-0258"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa062867"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.014.10146"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2010.07.001"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2018.1466294"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.143"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-047266"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-015-2314-2"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24275"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022110"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.16.5917-5929.2000"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2009.132"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2010.01.030"},{"key":"e_1_2_12_31_1","first-page":"267","article-title":"Evaluation of Maxwell(R) 16 for automated DNA extraction from whole blood and formalin\u2010fixed paraffin embedded (FFPE) tissue","volume":"50","author":"Khokhar S. K.","year":"2011","journal-title":"Clinical Chemistry and Laboratory Medicine"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6IB00127K"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth346"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899-012-0152-z"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0473-9-11"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0473-9-11"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01365.x"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2017.04.007"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V122.21.5163.5163"},{"key":"e_1_2_12_40_1","unstructured":"REN. (2012).Identification of DNA methylation biomarkers in imatinib\u2010resistant chronic myeloid leukemia cells."},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.139089"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-019-0729-2"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2016.104"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072298"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2039"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.9.2823"},{"key":"e_1_2_12_47_1","volume-title":"Human hg38 chr3:61561571\u201062297609 UCSC Genome Browser v390","author":"UCSC","year":"2013"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2010.187"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-017-0494-z"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i43.5692"},{"key":"e_1_2_12_51_1","first-page":"1405","article-title":"Methylation status of JunB and CDH13 gene promoter in CD34(+)CD38(\u2010) chronic myelogenous leukemia cells","volume":"17","author":"Wang X.","year":"2009","journal-title":"Zhongguo Shi Yan Xue Ye Xue Za Zhi"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-29-54"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28759"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S210168"}],"container-title":["Molecular Genetics &amp; Genomic Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmgg3.1319","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1319","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mgg3.1319","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1319","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T06:46:33Z","timestamp":1720075593000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mgg3.1319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,23]]},"references-count":53,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1002\/mgg3.1319"],"URL":"https:\/\/doi.org\/10.1002\/mgg3.1319","archive":["Portico"],"relation":{},"ISSN":["2324-9269","2324-9269"],"issn-type":[{"value":"2324-9269","type":"print"},{"value":"2324-9269","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,23]]},"assertion":[{"value":"2020-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1319"}}