{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:26:07Z","timestamp":1758925567470,"version":"3.41.2"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T00:00:00Z","timestamp":1607644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007403","name":"Dagmar Marshalls Fond","doi-asserted-by":"publisher","award":["500020"],"award-info":[{"award-number":["500020"]}],"id":[{"id":"10.13039\/100007403","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008208","name":"Overl\u00e6ge Johan Boserup og Lise Boserups Legat","doi-asserted-by":"publisher","award":["20795\u201024"],"award-info":[{"award-number":["20795\u201024"]}],"id":[{"id":"10.13039\/501100008208","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006576","name":"Civilingeni\u00f8r Frode V. Nyegaard og Hustrus Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006576","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006309","name":"Riisfort Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006309","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008264","name":"Karen A. Tolstrups Fond","doi-asserted-by":"publisher","award":["300.106\u2010012"],"award-info":[{"award-number":["300.106\u2010012"]}],"id":[{"id":"10.13039\/501100008264","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009512","name":"Agnes og Poul Friis Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009512","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010347","name":"Fabrikant Einar Willumsens Mindelegat","doi-asserted-by":"publisher","award":["6000073"],"award-info":[{"award-number":["6000073"]}],"id":[{"id":"10.13039\/501100010347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005361","name":"Grosserer Valdemar Foersom og Hustru Thyra Foersoms Fond","doi-asserted-by":"publisher","award":["41452\u2010001"],"award-info":[{"award-number":["41452\u2010001"]}],"id":[{"id":"10.13039\/501100005361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In gestational trophoblastic disease, the prognosis is related to the genetic constitution. In some cases, taking a biopsy is contraindicated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a pregnant woman, ultrasound scanning suggested hydatidiform mole. To explore if the genetic constitution can be established without taking a biopsy (or terminating the pregnancy), cell\u2010free DNA and circulating gestational trophoblasts were isolated from maternal blood before evacuation of the uterus. The evacuated tissue showed the morphology of a complete hydatidiform mole. Without prior whole\u2010genome amplification, short tandem repeat analysis of 24 DNA markers was performed on the samples, and on DNA isolated from evacuated tissue, and from the blood of the patient and her partner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Identical genetic results were obtained in each of three circulating gestational trophoblasts and the evacuated tissue, showing that this conceptus had a diploid androgenetic nuclear genome. In contrast, analysis of cell\u2010free DNA was less informative and less specific due to the inherent presence of cell\u2010free DNA from the patient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results show that it is possible to isolate and analyze circulating gestational trophoblasts originating in a pregnancy without maternal nuclear genome. For diagnosing gestational trophoblastic diseases, genotyping circulating gestational trophoblasts appears to be superior to analysis of cell\u2010free DNA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1565","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T20:10:06Z","timestamp":1607717406000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hydatidiform mole diagnostics using circulating gestational trophoblasts isolated from maternal blood"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8479-165X","authenticated-orcid":false,"given":"Lone","family":"Sunde","sequence":"first","affiliation":[{"name":"Department of Clinical Genetics Aalborg University Hospital Aalborg Denmark"},{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2657-0661","authenticated-orcid":false,"given":"Ripudaman","family":"Singh","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5428-8781","authenticated-orcid":false,"given":"Katarina","family":"Ravn","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8504-0893","authenticated-orcid":false,"given":"Palle","family":"Schelde","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7808-908X","authenticated-orcid":false,"given":"Estrid St\u00e6hr","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Pathology Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6449-6426","authenticated-orcid":false,"given":"Niels","family":"Uldbjerg","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s Disease and Birth Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isa","family":"Niemann","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s Disease and Birth Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-9172","authenticated-orcid":false,"given":"Lotte","family":"Hatt","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4429"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356073"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010019\u20100432\u20104"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4948"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000004721"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010016\u20101509\u20105"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12615"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.08.025"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.12.022"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142545"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0716"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2011.93"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5150"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2019.11.004"}],"container-title":["Molecular Genetics &amp; Genomic Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mgg3.1565","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mgg3.1565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:06:04Z","timestamp":1693199164000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mgg3.1565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,11]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/mgg3.1565"],"URL":"https:\/\/doi.org\/10.1002\/mgg3.1565","archive":["Portico"],"relation":{},"ISSN":["2324-9269","2324-9269"],"issn-type":[{"type":"print","value":"2324-9269"},{"type":"electronic","value":"2324-9269"}],"subject":[],"published":{"date-parts":[[2020,12,11]]},"assertion":[{"value":"2020-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1565"}}