{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T21:36:17Z","timestamp":1786397777481,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T00:00:00Z","timestamp":1612569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The alloimmunization following blood transfusion can be life\u2010threatening. The Rh alloantibodies are one of the most common causes contributing to alloimmunization. This study aimed to evaluate the rate and causes of alloimmunization and to determine the Rh phenotypes and genotypes among sickle cell disease (SCD) and sickle thalassemia (S\u03b2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Our study included 104 SCD and S\u03b2 patients referring to Baghaei 2 Hospital of Ahvaz in 2019 using a non\u2010random simple sampling method. The blood samples were collected for Rh phenotypes, alloantibody screening and identification, and molecular tests. The SSP\u2010PCR and RFLP methods with the Pst 1 enzyme were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The alloimmunization rate was 9.6% and 13.2% based on immunohematological tests and medical records, respectively. The main alloantibodies (90%) were anti\u2010Rh, and 40% of the patients had multiple alloantibodies. A significant correlation was found between gender and alloimmunization. The phenotypes of DCce (37.5%), DCcEe (24%), Dce (20.2%), and dce (5.8%) and genotypes of R1r (25%), R1R2 (20.2%), R1R1 (18.3%), and R1R0 (10.6%) were the most prevalent. The R1R2 was a frequent genotype in S\u03b2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>R0r\u2032 and R1R0 genotypes were limited to our population in Iran. Due to the differences in RH genotypes between our population and others, the blood transfusion from other ethnicities increased our total alloimmunization rate.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1614","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T18:05:27Z","timestamp":1612807527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7589-0325","authenticated-orcid":false,"given":"Mohammad Ali","family":"Jalali Far","sequence":"first","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arezoo","family":"Oodi","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naser","family":"Amirizadeh","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahshid","family":"Mohammadipour","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijan","family":"Keikhaei Dehdezi","sequence":"additional","affiliation":[{"name":"Thalassemia &amp; Hemoglobinopathy Research Center, Research Institute of Health Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,6]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"61","article-title":"Red blood cell alloimmunization among Sudanese homozygous sickle cell disease patients","volume":"3","author":"Abbas M.","year":"2013","journal-title":"American Journal of Medicine and Medical Sciences"},{"issue":"3","key":"e_1_2_9_3_1","first-page":"219","article-title":"Seroepidemiologic evaluation of Rh system major antigens (D, C, E, c, e) and their phenotypes among the blood donors in Khorramabad, Iran","volume":"6","author":"Abdi J.","year":"2009","journal-title":"The Scientific Journal of Iranian Blood Transfusion Organization (Khoon)"},{"issue":"4","key":"e_1_2_9_4_1","first-page":"42","article-title":"The incidence of ABO, Kell and Rh system blood groups in general population of Qazvin","volume":"9","author":"Ahmadi M. 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