{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:41Z","timestamp":1786978181178,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T00:00:00Z","timestamp":1613520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      An association between serum\u00a0vitamin D (Vit D) levels and systemic lupus erythematosus (SLE) has been reported by several studies that suggested the involvement of genetically determined characteristics of enzymes of vitamin D metabolism. Our study aimed to evaluate the relationship between 25 hydroxyvitamin D (25[OH]D) level, the most representative metabolite of VitD status, and polymorphism of the cytochrome P450,\n                      <jats:italic>CYP27B1<\/jats:italic>\n                      gene, which influence vitamin D metabolism, and serum levels, in SLE Tunisian patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study has been conducted in SLE patients (supplemented and not supplemented patients), matched to healthy controls by age and gender. The 25[OH]D serum level was measured by chemiluminescence assay and\n                      <jats:italic>CYP27B1<\/jats:italic>\n                      \u20101260 genetic polymorphism was carried out using PCR\u2010RFLP methods. Statistical analysis was made using Shesis and SPSS.20 Software.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Controls and Vit D not supplemented patients\u2019 groups presented the highest percentage of hypovitaminosis D. A significant difference in the mean level of circulating 25[OH]D between Vit D supplemented SLE patients and controls was observed (23.91\u00a0ng\/ml and 7.18\u00a0ng\/ml, respectively\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a03.4 10\n                      <jats:sup>5<\/jats:sup>\n                      ). Our results showed a correlation of high 25[OH]D level with complement\u00a0component 3 levels and prednisolone drug. Moreover, the analysis of\n                      <jats:italic>CYP27B1<\/jats:italic>\n                      \u20101260 polymorphism in SLE patients and controls revealed a nonsignificant allelic or genotypic association.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Despite the sunny climate, the high prevalence of Vit D deficiency is common in\u00a0Tunisia. This hypovitaminosis D feature may affect the Vit D levels in our SLE patients but a direct association with the disease or with the genetically determined features remains unclear. More studies are needed to establish thresholds and susceptibility genes according to the characteristics of each population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mgg3.1618","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T04:40:00Z","timestamp":1613796000000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Vitamin D status and\n                    <i>CYP27B<\/i>\n                    1\u20101260 promoter polymorphism in Tunisian patients with systemic lupus erythematosus"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5379-0622","authenticated-orcid":false,"given":"Raouia","family":"Fakhfakh","sequence":"first","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawsan","family":"Feki","sequence":"additional","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Elleuch","sequence":"additional","affiliation":[{"name":"Biochemistry Department Habib Bourguiba University Hospital Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"Neifar","sequence":"additional","affiliation":[{"name":"Biochemistry Department Habib Bourguiba University Hospital Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameh","family":"Marzouk","sequence":"additional","affiliation":[{"name":"Internal Medicine Department HediChaker University Hospital Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nesrine","family":"Elloumi","sequence":"additional","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hend","family":"Hachicha","sequence":"additional","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0208-145X","authenticated-orcid":false,"given":"Olfa","family":"Abida","sequence":"additional","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zouhir","family":"Bahloul","sequence":"additional","affiliation":[{"name":"Internal Medicine Department HediChaker University Hospital Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Ayadi","sequence":"additional","affiliation":[{"name":"Biochemistry Department Habib Bourguiba University Hospital Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatem","family":"Masmoudi","sequence":"additional","affiliation":[{"name":"Autoimmunity, Cancer and immunogenetics research laboratory University hospital Habib Bourguiba of Sfax Sfax Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncprheum0855"},{"issue":"1","key":"e_1_2_7_3_1","first-page":"56","article-title":"Vitamin D status in healthy Omani women of childbearing age: Study of female staff at the Royal Hospital, Muscat, Oman","volume":"11","author":"Al\u2010Kindi M. 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