{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:04:51Z","timestamp":1791846291678,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Next\u2010generation sequencing (NGS)\u2010based method is being used broadly for genetic testing especially for clinically and genetically heterogeneous disorders, such as inherited retinal degenerations (IRDs) but still not routinely used for molecular diagnostics in Bulgaria. Consequently, the purpose of this study was to evaluate the effectiveness of a molecular diagnostic approach, based on targeted NGS for the identification of the disease\u2010causing mutations in 16 Bulgarian patients with different IRDs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We applied a customized NGS panel, including 125 genes associated with retinal and other eye diseases to the patients with hereditary retinopathies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Systematic filtering approach coupled with copy number variation analysis and segregation study lead to the identification of 16 pathogenic and likely pathogenic variants in 12\/16 (75%) of IRD patients, 2 of which novel (12.5%): <jats:italic>ABCA4\u2010<\/jats:italic>c.668delA (p.K223Rfs18) and <jats:italic>R\u04201\u2010<\/jats:italic>c.2015dupA (p.K673Efs*25). Mutations in the <jats:italic>ABCA4, PRPH2, USH2A, BEST1, R\u04201, CDHR1<\/jats:italic>, and <jats:italic>RHO<\/jats:italic> genes were detected reaching a diagnostic yield between 42.9% for Retinitis pigmentosa cases and 100% for macular degeneration, Usher syndrome, and cone\u2010rod dystrophy patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results confirm the usefulness of targeted NGS approach based on frequently mutated genes as a comprehensive and successful genetic diagnostic tool for IRDs with significant impact on patients counseling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.1997","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T09:45:35Z","timestamp":1654249535000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Panel\u2010based next\u2010generation sequencing identifies novel mutations in Bulgarian patients with inherited 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