{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:01:47Z","timestamp":1753869707323,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["#2015A030313431"],"award-info":[{"award-number":["#2015A030313431"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Increasing evidence indicates that long non\u2010coding RNAs (lncRNAs) play crucial regulatory roles in epithelial\u2013mesenchymal transition (EMT). However, the regulatory mechanisms during EMT of the medial edge epithelium (MEE) remain elusive. The aim of this work is to reveal a novel lncRNA\u2010regulated dysfunction of EMT involved in the development of cleft palate (CP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57BL\/6\u00a0J mice at embryonic gestation day 14.5 (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 3 case samples vs. 3 control samples) were used to establish the CP model for lncRNA\u2013mRNA co\u2010expression profile analysis after high\u2010throughput sequencing. Functional predictions for the differentially expressed lncRNA\u2013mRNA co\u2010expression with transcription factor (TF)\u2010target gene relationship Gene Ontology\/Kyoto Encyclopedia of Genes and Genomes pathway (GO\/KEGG) analyses identified the regulatory \u201clncRNA\u2013TF\u2010target gene\u201d <jats:italic>trans <\/jats:italic>model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 583 differentially expressed lncRNAs and 703 differentially expressed mRNAs were identified. The results of <jats:italic>trans <\/jats:italic>analysis revealed that some TFs (<jats:italic>LEF1<\/jats:italic>, <jats:italic>SMAD4,<\/jats:italic> and <jats:italic>FOXD3<\/jats:italic>) regulate lncRNAs and gene expression. Finally, we identified the <jats:italic>NONMMUT034790.2\u2010LEF1\u2010SMAD7<\/jats:italic> co\u2010expression <jats:italic>trans<\/jats:italic>\u2010regulatory network that might be associated with CP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results revealed that <jats:italic>NONMMUT034790.2 <\/jats:italic>might be a novel epigenetic biomarker in CP. The integration of lncRNA modulators into <jats:italic>trans<\/jats:italic>\u2010regulatory networks will further enhance our understanding of lncRNA functions and regulatory mechanisms during palatal fusion in ATRA\u2010induced mouse CP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.522","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T04:55:07Z","timestamp":1544676907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A novel lncRNA\u2010mediated <i>trans<\/i>\u2010regulatory mechanism in the development of cleft palate in mouse"],"prefix":"10.1002","volume":"7","author":[{"given":"Xuan","family":"Shu","sequence":"first","affiliation":[{"name":"The Cleft Lip and Palate Treatment Center Second Affiliated Hospital of Shantou University Medical College Shantou 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