{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:48:29Z","timestamp":1776235709100,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,3]],"date-time":"2019-02-03T00:00:00Z","timestamp":1549152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Parkinson's disease (PD) is ranked as the second most common neurodegenerative disorder caused by loss of dopaminergic neurons in the substantia nigra. Micro(mi)RNAs are a class of small noncoding RNAs that regulate gene expression and aberrant expression of them is closely correlated with many neurodegenerative conditions including PD. Silent information regulator 1 (<jats:italic>SIRT1<\/jats:italic>) as a known deacetylase and B\u2010cell lymphoma\u20102 (<jats:italic>BCL2<\/jats:italic>) as an antiapoptotic factor play vital roles in neural protection and survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Differentiated PC12 cells exposed to MPP<jats:sup>+<\/jats:sup> were served here as a known PD model. Cell viability was determined by MTS assay. Apoptotic cells and ROS levels were detected using flow cytometry. Gene selection and miRNA\u2013mRNA interaction analysis were performed through in silico methods. Relative expression of miRNAs and genes was examined by RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MPP<jats:sup>+<\/jats:sup> exposure markedly reduced cell viability, enhanced oxidative stress, and induced apoptosis in differentiated PC12 cells. <jats:italic>Sirt1<\/jats:italic> and <jats:italic>BCL2<\/jats:italic>were shown to be markedly declined in response to MPP<jats:sup>+<\/jats:sup>, while miR\u2010200a and miR\u2010204 were significantly upregulated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The first novel finding of the current study is altered expression of miR\u2010200a and miR\u2010204 in differentiated PC12 cells in response to MPP<jats:sup>+<\/jats:sup>, suggesting that deregulation of them participate in MPP<jats:sup>+<\/jats:sup> neurotoxicity mechanisms, possibly via affecting the expression of <jats:italic>Sirt1<\/jats:italic> and <jats:italic>BCL2<\/jats:italic> as potential targets.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.548","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:43:53Z","timestamp":1549241033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Upregulation of miR\u2010200a and miR\u2010204 in MPP<sup>+<\/sup>\u2010treated differentiated PC12 cells as a model of Parkinson\u2019s disease"],"prefix":"10.1002","volume":"7","author":[{"given":"Maryam","family":"Talepoor Ardakani","sequence":"first","affiliation":[{"name":"Department of Biology, School of Sciences University of Isfahan Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahsa","family":"Rostamian Delavar","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences University of Isfahan Isfahan Iran"},{"name":"Department of Cellular Biotechnology, Cell Science Research Center Royan Institute for Biotechnology, ACECR Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoud","family":"Baghi","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences University of Isfahan Isfahan Iran"},{"name":"Department of Cellular Biotechnology, Cell Science Research Center Royan Institute for Biotechnology, ACECR Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hossein","family":"Nasr\u2010Esfahani","sequence":"additional","affiliation":[{"name":"Department of Cellular Biotechnology, Cell Science Research Center Royan Institute for Biotechnology, ACECR Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Kiani\u2010Esfahani","sequence":"additional","affiliation":[{"name":"Department of Cellular Biotechnology, Cell Science Research Center Royan Institute for Biotechnology, ACECR Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-5644","authenticated-orcid":false,"given":"Kamran","family":"Ghaedi","sequence":"additional","affiliation":[{"name":"Department of Biology, School of Sciences University of Isfahan Isfahan Iran"},{"name":"Department of Cellular Biotechnology, Cell Science Research Center Royan Institute for Biotechnology, ACECR Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-006-6715-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4803"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.04.010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.01.013"},{"issue":"8","key":"e_1_2_7_7_1","first-page":"697","article-title":"miRWalk2. 0: A comprehensive atlas of microRNA\u2010target interactions","volume":"12","author":"Dweep H.","year":"2015","journal-title":"NatureMethods"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-016-2764-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2012.09.006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.04.039"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"issue":"4","key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3233\/JPD-012144","article-title":"Plasma\u2010based circulating MicroRNA biomarkers for Parkinson's disease","volume":"2","author":"Khoo S. 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