{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:37:19Z","timestamp":1792521439690,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,10]],"date-time":"2019-04-10T00:00:00Z","timestamp":1554854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372228"],"award-info":[{"award-number":["81372228"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The serum tumor markers has been widely used in ovarian cancer diagnosis.\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      germline mutations are the most common predisposing factors for ovarian cancer development. This study aimed to comprehensively investigate serum tumor markers and\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      germline mutations and analyze their associations with ovarian cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Levels of 11 serum tumor markers were examined in ovarian cancer patients and controls with benign gynecologic diseases. By integrating multiplex PCR and next\u2010generation sequencing technologies,\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      germline mutations were analyzed and confirmed by Sanger sequencing. The discriminative models with serum tumor markers and\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      mutation status were constructed for ovarian cancer detection and patient stratification.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 11 markers, six of them were significantly elevated and only beta\u2010human chorionic gonadotropin (\u03b2\u2010HCG) was significantly reduced in ovarian cancer patients. A total of 54 (23.3%) ovarian cancer patients were found to harbor\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      deleterious mutations, and\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      mutations were significantly associated with Hereditary Breast and Ovarian Cancer\u2010related tumors and family history of cancer. Carbohydrate antigen 125 showed a good performance in ovarian cancer detection as a single marker (AUC\u00a0=\u00a00.799), while a panel of eight markers showed a good performance in BRCA1 mutation detection with an AUC value of 0.974. In addition, a panel of five serum tumor markers combined with\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      mutation status showed a good performance in lymph node metastasis prediction (AUC\u00a0=\u00a00.843).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We found the association between\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      germline mutation status and serum tumor marker levels, and identified discriminative models that combined serum tumor markers with\n                      <jats:italic>BRCA1\/2<\/jats:italic>\n                      mutation status for ovarian cancer detection and patient stratification.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mgg3.672","type":"journal-article","created":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T16:58:27Z","timestamp":1555001907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comprehensive analysis of serum tumor markers and\n                    <i>BRCA1\/2<\/i>\n                    germline mutations in Chinese ovarian cancer patients"],"prefix":"10.1002","volume":"7","author":[{"given":"Hongyu","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Xiangya School of Medicine Central South University Changsha China"},{"name":"Hunan Cancer Hospital &amp; The Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Sanway Gene Technology Inc. Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwu","family":"Guo","sequence":"additional","affiliation":[{"name":"Sanway Gene Technology Inc. Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfu","family":"Heng","sequence":"additional","affiliation":[{"name":"Hunan Cancer Hospital &amp; The Affiliated Cancer Hospital of Xiangya School of Medicine Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunxun","family":"Xu","sequence":"additional","affiliation":[{"name":"Sanway Gene Technology Inc. Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Peng","sequence":"additional","affiliation":[{"name":"Sanway Gene Technology Inc. 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