{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:56:21Z","timestamp":1774439781187,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013885","name":"Kementerian Kesihatan Malaysia","doi-asserted-by":"publisher","award":["NMRR\u201016\u20101468\u201032133"],"award-info":[{"award-number":["NMRR\u201016\u20101468\u201032133"]}],"id":[{"id":"10.13039\/501100013885","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp;amp; Gen Med"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>B\u2010cell precursor acute lymphoblastic leukemia (BCP\u2010ALL) is a prevalent pediatric hematologic malignancy characterized by diverse chromosomal aberrations that significantly influence its prognosis. This study aimed to comprehensively characterize the genomic landscape of BCP\u2010ALL in 55 Malaysian patients with BCP\u2010ALL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Single\u2010nucleotide polymorphism (SNP) 6.0 microarray and multiplex ligation\u2010dependent probe amplification were utilized to characterize and validate copy\u2010number abnormalities involving key oncogenes, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The SNP 6.0 microarray identified 191 copy\u2010number abnormalities in 55 patients, including common subtypes such as hyperdiploidy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914\/191, 7.3%), hypodiploidy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092\/191, 1.1%), and various copy\u2010number abnormalities such as interstitial (23.0%), terminal (17.2%), focal (37.1%), and intragenic (18.0%). Notably, intrachromosomal amplification of chromosome 21 (iAMP21) was not observed, suggesting its rarity in this cohort. Comparison with conventional cytogenetic techniques, including Trypsin\u2010Leishman's banding karyotyping, fluorescent in\u00a0situ hybridization (FISH), and reverse transcription\u2010polymerase chain reaction (RT\u2010PCR), revealed superior resolution of the SNP 6.0 microarray in detecting submicroscopic copy\u2010number abnormalities. Furthermore, MLPA confirmed abnormalities in several oncogenes, including\n                      <jats:italic>CDKN2A\/B<\/jats:italic>\n                      ,\n                      <jats:italic>EBF1<\/jats:italic>\n                      ,\n                      <jats:italic>ERG<\/jats:italic>\n                      ,\n                      <jats:italic>ETV6<\/jats:italic>\n                      ,\n                      <jats:italic>IKZF1<\/jats:italic>\n                      ,\n                      <jats:italic>JAK2<\/jats:italic>\n                      , and\n                      <jats:italic>PAX5<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study demonstrates the utility of combined SNP 6.0 microarray and MLPA in providing a comprehensive and refined understanding of the genetic landscape of BCP\u2010ALL in the Malaysian population. This understanding may facilitate risk stratification and the development of personalized treatment strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mgg3.70182","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:53:08Z","timestamp":1772981588000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single Nucleotide Polymorphism Microarray Analysis Unveils Copy\u2010Number Abnormalities and Genetic Heterogeneity in Malaysian Childhood B\u2010Cell Precursor Acute Lymphoblastic Leukemia"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1307-0097","authenticated-orcid":false,"given":"Nor Soleha","family":"Mohd Dali","sequence":"first","affiliation":[{"name":"Cancer Research Centre Institute for Medical Research, National Institutes of Health, Ministry of Health  Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7258-0687","authenticated-orcid":false,"given":"Nursaedah Abdullah","family":"Aziz","sequence":"additional","affiliation":[{"name":"Cancer Research Centre Institute for Medical Research, National Institutes of Health, Ministry of Health  Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3425-7632","authenticated-orcid":false,"given":"Muhamad Farid","family":"Zulkifle","sequence":"additional","affiliation":[{"name":"Cancer Research Centre Institute for Medical Research, National Institutes of Health, Ministry of Health  Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Durar Aqilah","family":"Zamri","sequence":"additional","affiliation":[{"name":"Genetics Department Hospital Tunku Azizah, Ministry of Health  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nor Rizan","family":"Kamaluddin","sequence":"additional","affiliation":[{"name":"Cancer Research Centre Institute for Medical Research, National Institutes of Health, Ministry of Health  Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seoh\u2010Leng","family":"Yeoh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Penang General Hospital, Ministry of Health  Penang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1973-9342","authenticated-orcid":false,"given":"Betty Lee\u2010Sue","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hospital Umum Sarawak, Ministry of Health  Sarawak Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazzlin Dizana","family":"Din","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hospital Sultanah Nur Zahirah, Ministry of Health  Terengganu Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azly Sumanty","family":"Ab Ghani","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Sultanah Nur Zahirah, Ministry of Health  Terengganu Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Amal Hayati","family":"Wan Hassan","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital Sultanah Nur Zahirah, Ministry of Health  Terengganu Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zubaidah","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Cytogenetics &amp; 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