{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:05:53Z","timestamp":1790168753669,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to screen and identify core hypoxia\u2010ischemia\u2010related genes associated with myocardial infarction (MI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Two transcriptomic datasets, GSE97320 and GSE48060, were retrieved from the Gene Expression Omnibus (GEO) database. After data integration and batch effect elimination, differential expression analysis was performed to screen differentially expressed genes (DEGs), and the corresponding visualization analysis was conducted. Hypoxia\u2010ischemia\u2010related genes were acquired from the GeneCards database; hypoxia\u2010ischemia related genes (HIRGs) were subsequently identified by intersecting the retrieved genes with screened DEGs. Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were implemented to explore the biological functions and underlying signaling pathways of HIRGs. A combination of protein\u2013protein interaction (PPI) network analysis and random forest (RF) algorithm was applied to screen hub genes from HIRGs. The external GEO dataset GSE66360 was utilized to validate the expression patterns of candidate hub genes. Furthermore, an acute myocardial infarction (AMI) mouse model was established, and quantitative real\u2010time polymerase chain reaction (qPCR) was performed to detect the mRNA expression levels of hub genes in myocardial tissues for in\u00a0vivo validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 633 DEGs and 308 hypoxia\u2010ischemia\u2010related genes were screened in the present study, among which 21 overlapping HIRGs were obtained. PLAUR and IL1B were finally identified as two hub genes from HIRGs based on PPI network and random forest algorithm. The qPCR results revealed that the expression levels of PLAUR and IL1B were significantly upregulated in the AMI group compared with the sham operation group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present findings demonstrated that PLAUR and IL1B serve as pivotal genes involved in the pathological hypoxia\u2010ischemia process of AMI. These two genes may act as novel biomarkers and promising therapeutic targets for the recognition and clinical intervention of hypoxia\u2010ischemia injury following AMI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mgg3.70284","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T07:16:21Z","timestamp":1788765381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioinformatics Analysis and Experimental Validation of Key Genes Associated With Hypoxia and Ischemia in Myocardial Infarction"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5810-1084","authenticated-orcid":false,"given":"Longsheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Ward 1, Department of Tuberculosis HIV\/AIDS Clinical Treatment Center of Guangxi (Nanning) and The Fourth People's Hospital of Nanning  Nanning Guangxi China"},{"name":"The First Affiliated Hospital of Guangxi Medical University  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2099-8782","authenticated-orcid":false,"given":"Ning","family":"Liang","sequence":"additional","affiliation":[{"name":"Ward 1, Department of Cardiovascular Medicine The Third People's Hospital of Nanning  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08008-5"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00538-y"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2025.21077"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202305581"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2007.03.005"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehaf032"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd12010006"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-24-0630"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-35548-z"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1267963"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehab405"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115554"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-024-02203-z"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bax028"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1094"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.633607"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.223925"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.tvir.2017.07.002"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2008.07.002"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI158788"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-022-02495-0"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI151268"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00647"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128296376240424072322"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2024.04.051"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.122.318333"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq675"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20164-6"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1330386"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.11.002"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203997"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1725381"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq537"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101460"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-025-03216-9"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.18591"}],"container-title":["Molecular Genetics &amp; 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